Skip Navigation



International Immunology Advance Access published online on May 20, 2008
International Immunology, doi:10.1093/intimm/dxn052
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Data
Right arrow All Versions of this Article:
20/7/937    most recent
dxn052v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Rifa'i, M.
Right arrow Articles by Suzuki, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rifa'i, M.
Right arrow Articles by Suzuki, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Japanese Society for Immunology. 2008. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

CD8+CD122+ regulatory T cells recognize activated T cells via conventional MHC class I–{alpha}βTCR interaction and become IL-10-producing active regulatory cells

Muhaimin Rifa'i1,2,*, Zhe Shi1,*, Shu-Yun Zhang3, Young Ho Lee1, Hiroshi Shiku4,5, Ken-ichi Isobe1 and Haruhiko Suzuki1

1 Department of Immunology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan
2 Department of Biology, Faculty of Science, Brawijaya University, Malang 65145, East-Java, Indonesia
3 Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan
4 Department of Cancer Vaccine
5 Department of Immuno-Gene Therapy, Mie University Graduate School of Medicine, Tsu 514-8507, Japan

Correspondence to: Correspondence to: H. Suzuki; E-mail: k46200a{at}nucc.cc.nagoya-u.ac.jp

CD8+CD122+ regulatory T cells (CD8+CD122+ Treg) are naturally occurring Treg that effectively suppress the proliferation and IFN-{gamma} production of both CD8+ and CD4+ target cells. This study investigated the molecular mechanisms of the recognition of target cells by CD8+CD122+ Treg using an in vitro culture system that reconstitutes the regulatory action of these cells. Naive CD8+CD122+ Treg co-cultured with pre-activated T cells became active Treg that produced IL-10 and suppressed IFN-{gamma} production from the target T cells. CD8+CD122+ Treg effectively suppressed the IFN-{gamma} production of the target cells of syngeneic mouse strains but not of allogeneic mouse strains with incompatible MHC. By using MHC-congeneic mouse strains, MHC-restricted suppression by CD8+CD122+ Treg was further confirmed. The blockade of cell surface molecules either on the Treg or on the target cells by specific blocking antibodies indicated that H-2K, H-2D, {alpha}βTCR and CD8 were involved in the regulatory action but I-A and Qa-1 were not. These results indicate that CD8+CD122+ Treg recognize already-activated T cells via the interaction of conventional MHC class I–{alpha}βTCR and become active regulatory cells that produce IL-10 and suppress the target cells.

Keywords: in vitro assay, mouse, suppression, Treg

* These authors contributed equally to this work

Transmitting editor: K. Okumura

Received 4 March 2008, accepted 25 April 2008.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.