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International Immunology Advance Access published online on March 7, 2008
International Immunology, doi:10.1093/intimm/dxn022
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© The Japanese Society for Immunology. 2008. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Application of CD27 as a marker for distinguishing human NK cell subsets

Anabel Silva1, Daniel M. Andrews1, Andrew G. Brooks2, Mark J. Smyth1 and Yoshihiro Hayakawa1,3

1 Cancer Immunology Program, Trescowthick Laboratories, Peter MacCallum Cancer Centre, St Andrews Place, East Melbourne, Victoria 3002, Australia
2 Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia
3 Present address: Pharmacology Department, Tsukuba Research Institute, Banyu Pharmaceutical Co. Ltd, 3 Okubo, Tsukuba, Ibaraki 300-2611, Japan

Correspondence to: Correspondence to: Y. Hayakawa; E-mail: yoshihiro_hayakawa{at}merck.com

It has long been recognized that human NK cells can be divided into two phenotypically and functionally distinct subsets, based on their levels of expression of CD56. We recently found that CD27 distinguishes subsets of mature mouse NK cells. Here we report that CD27 can be used as a marker to discriminate human NK cell subsets. The majority of peripheral blood human NK cells were CD27lo/CD56dim NK cells, whereas the minor CD27hi NK cell population correspondingly displayed a CD56bright phenotype. Distinctions between CD27lo and CD27hi NK cells in their receptor expression and typical NK cell functions such as cytotoxicity and cytokine production can be easily delineated. Therefore, we propose the dual use of CD27 and CD56 as maturation/subset markers for human NK cells. The identification of CD27 subsets in both mice and humans will allow more accurate projections of the role of NK cell subsets in murine models of human pathologies where NK cells are involved.

Keywords: human, NK cell, subset

Transmitting editor: K. Okumura

Received 1 October 2007, accepted 12 February 2008.


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