International Immunology Advance Access published online on October 26, 2006
International Immunology, doi:10.1093/intimm/dxl113
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawara-cho, Sakyo-ku, Kyoto 606-8507, Japan
* To whom correspondence should be addressed. Dendritic cells (DCs) and mast cells (MCs) co-localize in peripheral tissues of antigen entry, i.e. skin and mucosa. Due to the proximity of these two cell types, activation of MCs may affect DC functions. Here, we co-cultured human monocyte-derived DCs with cord blood-derived MCs activated by cross-linking of Fc
Received March 16, 2006
Accepted September 27, 2006
Article
IgE-activated mast cells in combination with pro-inflammatory factors induce Th2-promoting dendritic cells
Toshio Kitawaki 1, Norimitsu Kadowaki 1 *, Naoshi Sugimoto 1, Naotomo Kambe 2, Toshiyuki Hori 1, Yoshiki Miyachi 2, Tatsutoshi Nakahata 3, and Takashi Uchiyama 1
2 Department of Dermatology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawara-cho, Sakyo-ku, Kyoto 606-8507, Japan
3 Department of Pediatrics, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawara-cho, Sakyo-ku, Kyoto 606-8507, Japan
Norimitsu Kadowaki, E-mail: kadowaki{at}kuhp.kyoto-u.ac.jp
Abstract 
RI to elucidate the net effect of the whole MC products on DCs. Activated MCs induced maturation of DCs, and potently suppressed IL-12p70 production by the DCs. Whereas co-culture of DCs with activated MCs alone did not significantly influence the type of CD4+ T cell responses induced by the DCs, DCs co-cultured with activated MCs in the presence of pro-inflammatory or Th1-inducing factors caused Th2 polarization. Although histamine was involved in the induction of DC maturation and Th2 polarization by activated MCs, a combinatorial effect of various MC-derived factors, including those acting in a cell contact-dependent manner, was required for the optimal induction of Th2-promoting DCs. Furthermore, we demonstrated that clusters of DCs are located closely with MCs in lesions of atopic dermatitis. Collectively, this study suggests that the interaction between DCs and IgE-activated MCs in a pro-inflammatory or even Th1-prone environment is instrumental in maintaining and augmenting Th2 responses in allergy, and that disruption of the DC-MC interaction may constitute an effective strategy to treat ongoing allergic diseases.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Hiasa, M. Abe, A. Nakano, A. Oda, H. Amou, S. Kido, K. Takeuchi, K. Kagawa, K. Yata, T. Hashimoto, et al. GM-CSF and IL-4 induce dendritic cell differentiation and disrupt osteoclastogenesis through M-CSF receptor shedding by up-regulation of TNF-{alpha} converting enzyme (TACE) Blood, November 12, 2009; 114(20): 4517 - 4526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kambayashi, J. D. Baranski, R. G. Baker, T. Zou, E. J. Allenspach, J. E. Shoag, P. L. Jones, and G. A. Koretzky Indirect involvement of allergen-captured mast cells in antigen presentation Blood, February 1, 2008; 111(3): 1489 - 1496. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Christy and M. A. Brown The Multitasking Mast Cell: Positive and Negative Roles in the Progression of Autoimmunity J. Immunol., September 1, 2007; 179(5): 2673 - 2679. [Abstract] [Full Text] [PDF]
|
|