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International Immunology Advance Access published online on July 28, 2006
International Immunology, doi:10.1093/intimm/dxl072
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© The Japanese Society for Immunology. 2006. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org
Received August 12, 2005
Accepted July 5, 2006

Article

Identification of disulfide bonds in the Ig-{alpha}/Ig-{beta} component of the B cell antigen receptor using the Drosophila S2 cell reconstitution system

Gabrielle M. Siegers 1, Jianying Yang 1, Claudia U. Duerr 1, Peter J. Nielsen 1, Michael Reth 1, and Wolfgang W. A. Schamel 1 *

1 Max Planck-Institut für Immunbiologie and University of Freiburg, Biologie III Stübeweg 51, 79108 Freiburg, Germany

* To whom correspondence should be addressed.
Wolfgang W. A. Schamel, E-mail: schamel{at}immunbio.mpg.de


  Abstract

Structural information about immune receptor complexes is important for understanding signal transduction mechanisms. We have used the Drosophila S2 cell reconstitution system for identification of disulfide bonds within and between CD79a (Ig-{alpha}) and CD79b (Ig-{beta}), the heterodimeric signal transducing element of the B cell antigen receptor (BCR). Cysteines 113 and 135 of Ig-{alpha} and Ig-{beta}, respectively, form the intermolecular disulfide bridge stabilizing the Ig-{alpha}/Ig-{beta} heterodimer in both S2 cells and the B cell line J558L. Furthermore, using transfected S2 cells, two putative intramolecular disulfide bonds in the Ig-like domain of Ig-{beta} were identified. Ig-{beta}C65 and Ig-{beta}C120 form the canonical Ig fold disulfide bond. In addition, Ig-{beta}C43 and Ig-{beta}C124 also bind covalently. Individual cysteine to serine mutations in Ig-{alpha} had no influence on membrane-bound Ig (mIg)-M expression on the surface of S2 cells. In contrast, mIgM expression on the surface of B cells expressing Ig-{alpha}C113S was reduced, indicating that this intermolecular bond is prerequisite for efficient IgM-BCR formation. Our data also suggest that the Ig-{alpha}/Ig-{beta} heterodimer can assemble into oligomers.

Keywords: BCR assembly; cysteine mutants; Ig-{alpha}/Ig-{beta} heterodimer; Ig fold; surface mIgM expression.
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