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International Immunology Advance Access published online on May 30, 2006
International Immunology, doi:10.1093/intimm/dxl042
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© The Japanese Society for Immunology. 2006. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org
Received January 11, 2006
Accepted April 18, 2006

Article

The key residues in the immunodominant region 353-363 of human thyroid peroxidase were identified

Sandra A. Rebuffat 1, Damien Bresson 2, Brigitte Nguyen 1, and Sylvie Péraldi-Roux 1 *

1 CNRS UMR 5160, Faculté de Pharmacie, 34093 Montpellier Cedex 5, France
2 CNRS UMR 5160, Faculté de Pharmacie, 34093 Montpellier Cedex 5, France; Present address: Department of Developmental Immunology-3, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121, USA

* To whom correspondence should be addressed.
Sylvie Péraldi-Roux, E-mail: sylvie.roux{at}cpbs.univ-montp1.fr


  Abstract

Auto-antibodies (aAbs) to thyroid peroxidase (TPO) interact with a restricted immunodominant region (IDR) divided into two overlapping regions A and B. Among the five major regions structuring the IDR/B, regions 210-225, 353-363, 549-563, 713-720 and 766-775, region 353-363 constitutes an important anchor point for the binding of TPO-specific aAbs in sera from Hashimoto's and Graves' patients. We combined site-directed mutagenesis and expression of TPO mutants in stably transfected CHO cells to precisely define the critical residues in that region. By using flow cytometry and ELISA, we identified four amino acid residues, H353, D358, S359 and R361, that contribute to the interaction between human TPO and anti-TPO aAbs. This identification of these contributing amino acid residues in the IDR allowed us to more precisely depict contours of the IDR.

Keywords: auto-antibodies; autoimmune thyroid disease; autoimmunity; immunodominant region; thyroid peroxidase.
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