Skip Navigation



International Immunology Advance Access published online on March 28, 2006
International Immunology, doi:10.1093/intimm/dxl011
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
18/5/741    most recent
dxl011v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Caminschi, I.
Right arrow Articles by Wright, M. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Caminschi, I.
Right arrow Articles by Wright, M. D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Japanese Society for Immunology. 2006. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org
Received June 5, 2005
Accepted February 16, 2006

Article

Functional comparison of mouse CIRE/mouse DC-SIGN and human DC-SIGN

Irina Caminschi 1, Alexandra J. Corbett 2, Corina Zahra 3, Mireille Lahoud 3, Karen M. Lucas 1, Mariam Sofi 4, David Vremec 3, Thomas Gramberg 5, Stefan Pöhlmann 5, Joan Curtis 3, Emanuela Handman 3, Serani L. H. van Dommelen 6, Peter Fleming 6, Mariapia A. Degli-Esposti 6, Ken Shortman 1, and Mark D. Wright 4 *

1 Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia; Cooperative Research Centre for Vaccine Technology, Melbourne, Victoria 3050, Australia
2 Centre for Experimental Immunology, Lions Eye Institute, Western Australia
3 Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia
4 Mac Farlane Burnet Institute for Medical Research and Public Health, Kronheimer Building, A&RMC, Studley Road Heidelberg, Victoria 3084, Australia
5 Institute for Clinical and Molecular Virology and Nikolaus-Fiebiger Center, University of Erlangen-Nürnberg, 91054 Erlangen, Germany
6 Centre for Experimental Immunology, Lions Eye Institute, Western Australia; Immunology and Virology Program, Centre for Ophthalmology and Visual Science, University of Western Australia, Western Australia

* To whom correspondence should be addressed.
Mark D. Wright, E-mail: caminschi{at}wehi.edu.au


  Abstract

CIRE/mDC-SIGN is a C-type lectin we originally identified as a molecule differentially expressed by mouse dendritic cell (DC) populations. Immunostaining with a CIRE/mDC-SIGN-specific mAb revealed that CIRE/mDC-SIGN is indeed on the surface of some CD4+, CD4-8- DCs and plasmacytoid pre-DCs, but not on CD8+ DCs. It has been proposed that CIRE/mDC-SIGN is the functional orthologue of human DC-SIGN (hDC-SIGN), a molecule that both enhances T cell responses and facilitates antigen uptake. We assessed if CIRE/mDC-SIGN and hDC-SIGN exhibit functional similarities. CIRE/mDC-SIGN is down-regulated upon activation, but unlike hDC-SIGN, incubation with IL-4 and IL-13 did not enhance CIRE/mDC-SIGN expression, indicating differences in gene regulation. Like hDC-SIGN, CIRE/mDC-SIGN bound mannosylated residues. However, we could detect no role for CIRE/mDC-SIGN in T cell-DC interactions and the protein did not bind to pathogens known to interact with hDC-SIGN, including Leishmania mexicana, cytomegalovirus, HIV and lentiviral particles bearing the Ebolavirus glycoprotein. The binding of CIRE/mDC-SIGN to hDC-SIGN ligands was not rescued when CIRE/mDC-SIGN was engineered to express the stalk region of hDC-SIGN. We conclude that there are significant differences in the fine specificity of the C-type lectin domains of hDC-SIGN and CIRE/mDC-SIGN and that these two molecules may not be functional orthologues.

Keywords: cell-surface molecules; dendritic cells; rodents.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 JEMHome page
A. Tanne, B. Ma, F. Boudou, L. Tailleux, H. Botella, E. Badell, F. Levillain, M. E. Taylor, K. Drickamer, J. Nigou, et al.
A murine DC-SIGN homologue contributes to early host defense against Mycobacterium tuberculosis
J. Exp. Med., September 28, 2009; 206(10): 2205 - 2220.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Srivastava, M. Manchanda, S. Gupta, R. Singla, D. Behera, G. Das, and K. Natarajan
Toll-like Receptor 2 and DC-SIGNR1 Differentially Regulate Suppressors of Cytokine Signaling 1 in Dendritic Cells during Mycobacterium tuberculosis Infection
J. Biol. Chem., September 18, 2009; 284(38): 25532 - 25541.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
I. Caminschi, A. I. Proietto, F. Ahmet, S. Kitsoulis, J. Shin Teh, J. C. Y. Lo, A. Rizzitelli, L. Wu, D. Vremec, S. L. H. van Dommelen, et al.
The dendritic cell subtype-restricted C-type lectin Clec9A is a target for vaccine enhancement
Blood, October 15, 2008; 112(8): 3264 - 3273.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
I. Caminschi, F. Ahmet, K. Heger, J. Brady, S. L. Nutt, D. Vremec, S. Pietersz, M. H. Lahoud, L. Schofield, D. S. Hansen, et al.
Putative IKDCs are functionally and developmentally similar to natural killer cells, but not to dendritic cells
J. Exp. Med., October 29, 2007; 204(11): 2579 - 2590.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.