Skip Navigation



International Immunology Advance Access published online on February 28, 2006
International Immunology, doi:10.1093/intimm/dxh400
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
18/4/581    most recent
dxh400v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Samara, M.
Right arrow Articles by Khamlichi, A. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Samara, M.
Right arrow Articles by Khamlichi, A. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Japanese Society for Immunology. 2006. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org
Received August 31, 2005
Accepted January 17, 2006

Article

Germ line transcription in mice bearing neor gene downstream of I{gamma}3 exon in the Ig heavy chain locus

Maha Samara 1, Zeliha Oruc 2, Hei-Lanne Dougier 2, Tamer Essawi 3, Michel Cogné 4, and Ahmed Amine Khamlichi 5 *

1 CNRS UMR 6101, Groupe ‘Instabilité génétique et régulation transcriptionnelle’, Faculté de Médecine, 2 rue du Dr Marcland, F-87025 Limoges cedex, France; Department of Clinical Sciences, Birzeit University, Birzeit, Palestine
2 CNRS UMR 6101, Groupe ‘Instabilité génétique et régulation transcriptionnelle’, Faculté de Médecine, 2 rue du Dr Marcland, F-87025 Limoges cedex, France
3 Department of Clinical Sciences, Birzeit University, Birzeit, Palestine
4 CNRS UMR 6101, Groupe ‘Génétique moléculaire de la cellule B et des syndromes immunoprolifératifs’, Faculté de Médecine, Limoges, France
5 CNRS UMR 6101, Groupe ‘Instabilité génétique et régulation transcriptionnelle’, Faculté de Médecine, 2 rue du Dr Marcland, F-87025 Limoges cedex, France; Present address: CNRS UMR 5089-IPBS, Equipe "Instabilité génétique et régulation transcriptionnelle", 205 route de Narbonne, 31077 Toulouse cedex 4, France

* To whom correspondence should be addressed.
Ahmed Amine Khamlichi, E-mail: ahmed.khamlichi{at}unilim.fr


  Abstract

Class switch recombination (CSR) is preceded by germ line transcription that initiates from promoters upstream of switch (S) sequences and terminates downstream of associated constant genes. Previous work showed that germ line transcripts and their processing are required for CSR and that germ line transcription is regulated in a major part by a regulatory region located downstream of the Ig heavy chain locus. This long-range, polarized effect can be disturbed by inserting an expressed neomycine resistance (neor) gene. To contribute to a better understanding of the mechanism of such a long-distance regulation, we generated knock-in mice in which a neor gene was inserted downstream of I{gamma}3 exon leaving intact all the necessary elements for germ line transcription and splicing. We show that the expressed neor gene interferes with transcription initiation from I{gamma}3, and that it impairs but does not block S recombination to C{gamma}3. Moreover, we show for the first time that the neor gene provides through chimeric neor-C{gamma}3 transcripts the necessary elements for splicing of germ line transcripts by activating two novel cryptic splice sites, one in the coding region of the intronless neor gene and the other in the I{gamma}3-C{gamma}3 intron.

Keywords: B lymphocyte; class switch recombination; long-range interaction; splicing.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
Z. Oruc, A. Boumediene, M. Le Bert, and A. A. Khamlichi
Replacement of I{gamma}3 germ-line promoter by I{gamma}1 inhibits class-switch recombination to IgG3
PNAS, December 18, 2007; 104(51): 20484 - 20489.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.