International Immunology Advance Access published online on January 12, 2006
International Immunology, doi:10.1093/intimm/dxh369
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1 Center for Genomic Medicine, Graduate School of Medicine Kyoto University, Kyoto, Japan; Division of Systemic Life Sciences, Graduate School of Biostudies, Kyoto University, Kyoto, Japan
* To whom correspondence should be addressed. Stromal cells in lymphoid tissues provide microenvironmental fields required for the triggering of efficient immune responses. Fibroblastic reticular cells (FRCs) are one of the integral constituents of such stromal fields; they construct the reticular network and are considered to regulate immune cells' behavior. However, the factors that mediate the interaction between lymphocytes and FRCs are poorly understood. Here we show that a mouse lymph node (LN)-derived FRC cell line, BLS4, expresses a transmembrane chemokine, CXC chemokine ligand (CXCL) 16, in response to tumor necrosis factor
Received August 24, 2005
Accepted November 16, 2005
Article
A transmembrane chemokine, CXC chemokine ligand 16, expressed by lymph node fibroblastic reticular cells has the potential to regulate T cell migration and adhesion
Takahiro Hara 1,
Tomoya Katakai 2 *,
Jong-Hwan Lee 1,
Yukiko Nambu 1,
Natsuki Nakajima-Nagata 3,
Hiroyuki Gonda 4,
Manabu Sugai 3,
and
Akira Shimizu 5
2 Center for Genomic Medicine, Graduate School of Medicine Kyoto University, Kyoto, Japan; Present address: 53 Shogoin-Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan
3 Center for Genomic Medicine, Graduate School of Medicine Kyoto University, Kyoto, Japan
4 Center for Genomic Medicine, Graduate School of Medicine Kyoto University, Kyoto, Japan; Translational Research Center, Kyoto University Hospital, Kyoto 606-8507, Japan
5 Center for Genomic Medicine, Graduate School of Medicine Kyoto University, Kyoto, Japan; Division of Systemic Life Sciences, Graduate School of Biostudies, Kyoto University, Kyoto, Japan; Translational Research Center, Kyoto University Hospital, Kyoto 606-8507, Japan
Tomoya Katakai, E-mail: tkatakai{at}virus.kyoto-u.ac.jp
Abstract 
(TNF) and IFN
. TNF-induced expression of CXCL16 depends on NF
B, p38 MAPK and PKA. Matrix metalloproteinase activity is required for producing soluble CXCL16 in the culture supernatant, likely via shedding at the juxtamembrane region of the extracellular domain. IL-12 enhances the expression of CXCR6 in anti-CD3/CD28-stimulated CD8+ T cells and their adhesion to the BLS4 cell surface in a TNF-dependent fashion. The adherence is significantly inhibited in the presence of both anti-CXCL16 and anti-vascular cell adhesion molecule 1 (VCAM-1) antibodies. CXCL16 expression is also detected in the FRCs in LN sections and in gp38+VCAM-1+ FRCs isolated from LNs. Taken together, these findings suggest that CXCL16 is an important mediator of lymphocyte-stromal interaction within lymphoid tissues.
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