International Immunology Advance Access published online on November 15, 2005
International Immunology, doi:10.1093/intimm/dxh348
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Cancer Immunology and Immunotherapy Center, Saint Savas Hospital, 171 Alexandras Avenue, Athens 115 22, Greece
* To whom correspondence should be addressed. IL-21 plays a role in the proliferation and maturation of NK cells developed from hematopoietic stem cells. In this study, we found that IL-21, in the presence of physiological concentration of hydrocortisone (HC), has a significant impact on the functions of NK cells derived from umbilical cord blood (CB) populations. We demonstrate that IL-21, in combination with Flt3-ligand, IL-15 and HC, induces high proliferative responses and, apart from enhancing NK-mediated cytotoxicity, it also induces a significant increase in lymphokine-activated killer activity of CB/CD34+-derived CD56+ cells. In addition, IL-21 induced changes in the CD56+ cell cytokine secretion profile. Thus, we observed increased levels of IL-10 and granulocyte macrophage colony-stimulating factor, whereas tumor necrosis factor-
Received December 14, 2004
Accepted October 4, 2005
Article
Effect of IL-21 on NK cells derived from different umbilical cord blood populations
Sonia A. Perez 1 *,
Louisa G. Mahaira 1,
Panagiota A. Sotiropoulou 1,
Angelos D. Gritzapis 1,
Eleni G. Iliopoulou 1,
Dimitrios K. Niarchos 1,
Nike T. Cacoullos 1,
Yannis G. Kavalakis 2,
Aris I. Antsaklis 2,
Nectaria N. Sotiriadou 1,
Constantin N. Baxevanis 1,
and
Michael Papamichail 1
2 1st Obstetrics and Gynecology University Clinic, Alexandras Maternity Hospital, Athens, Greece
Sonia A. Perez, E-mail: perez{at}ciic.gr
Abstract 
levels decreased. IFN-
production was also modified by IL-21, depending on the presence or absence of IL-18. CB/CD34+ cells did not express the IL-21R ex vivo, but receptor expression was induced during their commitment to differentiation into CD56+ cells. Our data ascribe to IL-21 an essential role on NK cell development and function under conditions similar to the in vivo CB microenvironment.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  G. Zhang, J. Rowe, M. Kusel, A. Bosco, K. McKenna, N. de Klerk, P. D. Sly, and P. G. Holt Interleukin-10/Interleukin-5 Responses at Birth Predict Risk for Respiratory Infections in Children with Atopic Family History Am. J. Respir. Crit. Care Med., February 1, 2009; 179(3): 205 - 211. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Vitale, F. Cottalasso, E. Montaldo, L. Moretta, and M. C. Mingari Methylprednisolone induces preferential and rapid differentiation of CD34+ cord blood precursors toward NK cells Int. Immunol., April 1, 2008; 20(4): 565 - 575. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. D. Davis, K. Skak, M. J. Smyth, P. E.G. Kristjansen, D. M. Miller, and P. V. Sivakumar Interleukin-21 Signaling: Functions in Cancer and Autoimmunity Clin. Cancer Res., December 1, 2007; 13(23): 6926 - 6932. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Good, V. L. Bryant, and S. G. Tangye Kinetics of Human B Cell Behavior and Amplification of Proliferative Responses following Stimulation with IL-21 J. Immunol., October 15, 2006; 177(8): 5236 - 5247. [Abstract] [Full Text] [PDF]
|
|