Skip Navigation



International Immunology Advance Access published online on November 1, 2005
International Immunology, doi:10.1093/intimm/dxh340
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
17/12/1607    most recent
dxh340v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kao, C.
Right arrow Articles by Jameson, S. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kao, C.
Right arrow Articles by Jameson, S. C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Japanese Society for Immunology. 2005. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org
Received September 14, 2005
Accepted September 22, 2005

Article

Loss of CD8 and TCR binding to Class I MHC ligands following T cell activation

Charlly Kao 1, Mark A. Daniels 2, and Stephen C. Jameson 1*

1 Department of Laboratory Medicine and Pathology, Center for Immunology, University of Minnesota, MMC 334, 420 Delaware Street SE, Minneapolis, MN 55455, USA
2 Department of Laboratory Medicine and Pathology, Center for Immunology, University of Minnesota, MMC 334, 420 Delaware Street SE, Minneapolis, MN 55455, USA; Present address: Department of Transplantation Immunology and Nephrology, University Hospital Basel, CH 4031 Basel, Switzerland

* To whom correspondence should be addressed.
Stephen C. Jameson, E-mail: james024{at}umn.edu


  Abstract

The capacity of T cells to bind peptide/MHC ligands changes with T cell development and differentiation. Here we study changes in peptide/MHC multimer binding following T cell activation. Surprisingly, T cell activation caused a marked reduction in specific peptide/MHC Class I multimer binding, which was distinct from transient TCR down-regulation, and was especially dramatic for engagement with low-affinity peptide/MHC ligands. Direct CD8-Class I interactions were also profoundly and rapidly impaired following T cell stimulation, even though surface CD8{alpha} and CD8{beta} levels were unchanged after activation, suggesting that decreased CD8 co-receptor binding contributes to this effect. Finally, we show that enzymatic desialylation restores much of the multimer binding on activated T cells, suggesting that altered glycosylation may inhibit TCR/CD8 binding to peptide/MHC ligands. These radical changes in activated T cells' ability to perceive peptide/MHC ligands may contribute to selective outgrowth of clones with high affinity for the stimulatory ligand.

Keywords: activation; CTL; tetramer.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
D. Thakral, J. Dobbins, L. Devine, and P. B. Kavathas
Differential Expression of the Human CD8{beta} Splice Variants and Regulation of the M-2 Isoform by Ubiquitination
J. Immunol., June 1, 2008; 180(11): 7431 - 7442.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
Z. Xiao, M. F. Mescher, and S. C. Jameson
Detuning CD8 T cells: down-regulation of CD8 expression, tetramer binding, and response during CTL activation
J. Exp. Med., October 29, 2007; 204(11): 2667 - 2677.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Kao, M. M. Sandau, M. A. Daniels, and S. C. Jameson
The Sialyltransferase ST3Gal-I Is Not Required for Regulation of CD8-Class I MHC Binding during T Cell Development.
J. Immunol., June 15, 2006; 176(12): 7421 - 7430.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.