Skip Navigation



International Immunology Advance Access published online on November 22, 2005
International Immunology, doi:10.1093/intimm/dxh335
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
17/12/1561    most recent
dxh335v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Rubio, M. T.
Right arrow Articles by Sykes, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rubio, M. T.
Right arrow Articles by Sykes, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Japanese Society for Immunology. 2005. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org
Received March 23, 2005
Accepted September 15, 2005

Article

Maturation of human monocyte-derived dendritic cells (MoDCs) in the presence of prostaglandin E2 optimizes CD4 and CD8 T cell-mediated responses to protein antigens: role of PGE2 in chemokine and cytokine expression by MoDCs

Marie T. Rubio 1, Terry K. Means 2, Ronjon Chakraverty 1, Juanita Shaffer 1, Yasuhiro Fudaba 1, Meredith Chittenden 1, Andrew D. Luster 2, and Megan Sykes 1 *

1 Bone Marrow Transplantation Section, Transplantation Biology Research Center, Massachusetts General Hospital and Harvard Medical School, MGH-East, Building 149-5102, 13th Street, Boston, 02129, USA
2 Center for Immunology and Inflammatory Diseases and Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02129, USA

* To whom correspondence should be addressed.
Megan Sykes, E-mail: megan.sykes{at}tbrc.mgh.harvard.edu


  Abstract

Prostaglandin E2 (PGE2) acts in synergy with other inflammatory stimuli such as tumor necrosis factor (TNF) to induce the maturation of migratory-type monocyte-derived dendritic cells (MoDCs). However, PGE2 has been reported to inhibit IL-12p70 production by MoDCs and to promote the generation of Th2 T cell responses. We demonstrate here that the addition of PGE2 to TNF for the maturation of MoDCs enhanced CD4 and CD8 T cell proliferative responses to neoantigen and recall antigen, and enhanced Th1-type responses. The increased stimulatory capacity of MoDCs matured with PGE2 was associated with a fully mature, migratory-type MoDC phenotype and more rapid down-regulation of the expression of inflammatory chemokines, with up-regulated expression of the constitutive chemokines TARC and MDC. In addition, although MoDCs matured with TNF and PGE2 selectively produced the inhibitory IL-12p40 subunit at steady state, they were able to produce the bioactive IL-12p70 heterodimer after stimulation with CD40 ligand and/or IFN-{gamma}. Despite increased IL-6 mRNA expression, MoDCs matured with PGE2 did not overcome the suppressive effects of CD4+CD25+ T cells in allogeneic mixed lymphocyte reactions. In conclusion, MoDCs matured in the presence of PGE2 display characteristics of more efficient antigen-presenting cells that might be optimal for use in cancer vaccine-based clinical trials.

Keywords: dendritic cells; human; prostaglandin E2; T helper 1; T helper 2.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 BloodHome page
P. Krause, M. Bruckner, C. Uermosi, E. Singer, M. Groettrup, and D. F. Legler
Prostaglandin E2 enhances T-cell proliferation by inducing the costimulatory molecules OX40L, CD70, and 4-1BBL on dendritic cells
Blood, March 12, 2009; 113(11): 2451 - 2460.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. Agaugue, E. Marcenaro, B. Ferranti, L. Moretta, and A. Moretta
Human natural killer cells exposed to IL-2, IL-12, IL-18, or IL-4 differently modulate priming of naive T cells by monocyte-derived dendritic cells
Blood, September 1, 2008; 112(5): 1776 - 1783.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Khayrullina, J.-H. Yen, H. Jing, and D. Ganea
In Vitro Differentiation of Dendritic Cells in the Presence of Prostaglandin E2 Alters the IL-12/IL-23 Balance and Promotes Differentiation of Th17 Cells
J. Immunol., July 1, 2008; 181(1): 721 - 735.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. F. G. van Helden, M. M. Oud, B. Joosten, N. Peterse, C. G. Figdor, and F. N. van Leeuwen
PGE2-mediated podosome loss in dendritic cells is dependent on actomyosin contraction downstream of the RhoA-Rho-kinase axis
J. Cell Sci., April 1, 2008; 121(7): 1096 - 1106.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
P. Krause, E. Singer, P. I. Darley, J. Klebensberger, M. Groettrup, and D. F. Legler
Prostaglandin E2 is a key factor for monocyte-derived dendritic cell maturation: enhanced T cell stimulatory capacity despite IDO
J. Leukoc. Biol., November 1, 2007; 82(5): 1106 - 1114.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.