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International Immunology Advance Access published online on August 15, 2005
International Immunology, doi:10.1093/intimm/dxh301
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© The Japanese Society for Immunology. 2005. All rights reserved. For permissions, please e-mail: journals.permissions@oupjournals.org
Received March 4, 2005
Accepted June 29, 2005

Article

IgE- and Fc{varepsilon}RI-mediated migration of human basophils

Maho Suzukawa 1, Koichi Hirai 2, Motoyasu Iikura 1, Hiroyuki Nagase 3, Akiko Komiya 1, Chitose Yoshimura-Uchiyama 4, Hirokazu Yamada 1, Chisei Ra 5, Ken Ohta 3, Kazuhiko Yamamoto 1, and Masao Yamaguchi 1*

1 Department of Allergy and Rheumatology, University of Tokyo Graduate School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan
2 Department of Bioregulatory Function, University of Tokyo Graduate School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan
3 Department of Respiratory Medicine, University of Teikyo School of Medicine, Tokyo, Japan
4 Department of Allergy and Rheumatology, University of Tokyo Graduate School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan; Department of Pediatrics, University of Tokyo Graduate School of Medicine, Tokyo, Japan
5 Division of Molecular Cell Immunology and Allergology, Nihon University Graduate School of Medical Sciences, Tokyo, Japan

* To whom correspondence should be addressed.
Masao Yamaguchi, E-mail: myama-tky{at}umin.ac.jp


  Abstract

Local accumulation of basophils at inflammatory sites is observed in experimental antigen challenge and in allergic diseases. It is not fully known what factor(s) regulates local basophil influx in tissues, and it has not been determined whether antigens belong in a panel of basophil chemoattractants. This study was designed to elucidate whether IgE- and high-affinity receptor for IgE (Fc{varepsilon}RI)-mediated stimulation can induce human basophil migration. The migration-inducing potency of an anti-Fc{varepsilon}RI {alpha}-chain mAb, CRA-1, was examined on human basophils. CRA-1 mAb elicited significant migration of basophils. The migration-inducing potency of this mAb was maximal at 100 ng ml-1, and CRA-1 mAb at 100 ng ml-1 attracted ~10% of total inoculated basophils above baseline levels after incubation for 2.5 h. Checkerboard analysis indicated that basophil migration induced by this mAb was mainly chemotactic and partially chemokinetic. An antigen, Der f 2, also induced migration of basophils from Der f-sensitive subjects. Basophils mixed with 1 ng ml-1 of CRA-1 mAb showed an exaggerated migration response to eotaxin, indicating that Fc{varepsilon}RI cross-linkage enhances basophil migration to other chemoattractants. Induction of basophil migration by IgE- and Fc{varepsilon}RI-cross-linking stimulation may, at least in part, explain the pathogenesis of local basophil accumulation clinically observed in allergic diseases such as asthma.

Keywords: allergy; antigen; chemotaxis; non-releaser.
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M. Suzukawa, A. Komiya, M. Iikura, H. Nagase, C. Yoshimura-Uchiyama, H. Yamada, H. Kawasaki, K. Ohta, K. Matsushima, K. Hirai, et al.
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[Abstract] [Full Text] [PDF]


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