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International Immunology Advance Access published online on July 18, 2005
International Immunology, doi:10.1093/intimm/dxh296
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© The Japanese Society for Immunology. 2005. All rights reserved. For permissions, please e-mail: journals.permissions@oupjournals.org
Received March 23, 2005
Accepted June 13, 2005

Article

Allogeneic T lymphocytes as a source of peptide-dependent T cells specific for myeloma cells

Alexandrine Geffroy-Luseau 1, Agnès Moreau-Aubry 1, Régis Bataille 1, and Catherine Pellat-Deceunynck 1*

1 Inserm UMR601, Institut de Biologie, 9 quai Moncousu, 44093 Nantes cedex 01, France

* To whom correspondence should be addressed.
Catherine Pellat-Deceunynck, E-mail: catherine.pellat-deceunynck{at}univ-nantes.fr


  Abstract

We investigated the generation of myeloma-specific CTLs from normal donors HLA mismatched with the myeloma cell line SBN. The T-cell line obtained was cloned and each CTL was assessed against SBN and SBN-EBV (a B-EBV cell line obtained by EBV infection of B cells from SBN patient) simultaneously. Among >270 clones evaluated, 2 CTLs (Vbeta13.1 and Vbeta17) killed SBN but spared SBN-EBV cells. Antibodies against HLA-I, but not HLA-A2, molecules abrogated their recognition of SBN. Moreover, SBN recognition was abrogated by anti-HLA-Cw6 antiserum. Both clones recognized two other HLA-Cw*0602 myeloma cell lines. Neither of them recognized HLA-Cw*0602 B-EBV cell lines, the PBMCs of HLA-Cw*0602-unrelated donors or HLA-Cw*0602 melanoma cell lines. We showed that HLA-Cw6 molecules were more expressed at the cell surface of B-EBV cells as compared with myeloma cells, suggesting that the lack of reactivity against B-EBV cells was not related to a low level of HLA expression. Since CTL clones did not express any KIR or NKG2D, we excluded the fact that NK cell receptors could be involved in myeloma-specific recognition through KIR-HLA-I or NKG2D-MICA,B interactions. Cold target competition and acid elution experiments confirmed that myeloma cell recognition was peptide dependent.

Keywords: multiple myeloma; CTL.
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