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International Immunology Advance Access published online on March 3, 2005
International Immunology, doi:10.1093/intimm/dxh228
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© The Japanese Society for Immunology. 2005. All rights reserved. For permissions, please e-mail: journals.permissions@oupjournals.org
Received July 27, 2004
Accepted January 27, 2005

Article

CD83 localization in a recycling compartment of immature human monocyte-derived dendritic cells

Elisabeth Klein 1 *, Susanne Koch 1* *, Bodo Borm 2, Jürgen Neumann 3, Volker Herzog 2, Norbert Koch 3, and Thomas Bieber 1

1 Department of Dermatology, Friedrich-Wilhelms-University of Bonn, Sigmund-Freud-Strasse 25, D-53105 Bonn, Germany
2 Department of Cell Biology, Friedrich-Wilhelms-University of Bonn, Sigmund-Freud-Strasse 25, D-53105 Bonn, Germany
3 Institute of Molecular Physiology, Division of Immunobiology, Friedrich-Wilhelms-University of Bonn, Roemerstrasse 164, D-53105 Bonn, Germany

* To whom correspondence should be addressed.
Susanne Koch, E-mail: susanne.koch{at}ukb.uni-bonn.de


  Abstract

Dendritic cells (DC) change their phenotype and functional properties during maturation. CD83 cell surface expression is induced on mature DC (mDC). In this study, we investigated intracellular CD83 localization and transport in human monocyte-derived DC. The enhanced level of CD83 cell surface expression in mDC resulted predominantly from increased protein synthesis, and in addition from regulated intracellular transport of CD83 protein. An internal pool of CD83 protein is present in immature DC (iDC). Although CD83 protein in iDC and in mDC was localized in the Golgi compartment and in recycling endosomes, only in mature cells did CD83 co-localize with MHC class II molecules in endocytic vesicles. CD83 cell surface expression on iDC was induced by inhibition of endocytosis. This result could be explained by CD83 cycling between endosomes and the cell surface in iDC. The mDC also rapidly internalized membrane-bound CD83 protein. Furthermore, a thiol protease inhibitor and specific cathepsin inhibitors impaired CD83 up-regulation in DC, indicating a role of endosomal proteases in the maturation-induced exposure of CD83 on the plasma membrane.

Keywords: antigen presentation; dendritic cells; differentiation marker; endosomes.

*These authors contributed equally to this work.


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