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International Immunology Advance Access published online on December 27, 2004
International Immunology, doi:10.1093/intimm/dxh191
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© 2005 The Japanese Society for Immunology
Received September 29, 2004
Accepted October 27, 2004

Article

The non-classical HLA class I molecule HFE does not influence the NK-like activity contained in fresh human PBMCs and does not interact with NK cells

Steve Pascolo 1*, Florent Ginhoux 2, Nihay Laham 3, Steffen Walter 1, Oliver Schoor 1, Jochen Probst 1, Pierre Rohrlich 4, Florian Obermayr 1, Paul Fisch 5, Olivier Danos 2, Rachel Ehrlich 3, Francois A. Lemonnier 6, and Hans-Georg Rammensee 1

1 Department of Immunology, Auf der Morgenstelle 15, 72076 Tübingen, Germany
2 Genethon CNRS UMR 8115, 1 bis rue de l'Internationale, BP60, Evry Cedex 91002, France
3 Department of Cell Research and Immunology, Tel Aviv University, Tel Aviv, Israel
4 Immunité Cellulaire Antivirale, Institut Pasteur, 28 rue du Dr. Roux, 75715 Paris, France; Hospital Robert Debré, 75019 Paris, France
5 Pathologisches Institut, University of Freiburg, Freiburg, Germany
6 Immunité Cellulaire Antivirale, Institut Pasteur, 28 rue du Dr. Roux, 75715 Paris, France

* To whom correspondence should be addressed.
Steve Pascolo, E-mail: steve.pascolo{at}uni-tuebingen.de


  Abstract

In humans, four {beta}2-microglobulin-associated non-classical class I molecules are encoded in the MHC: HLA-E, -F, -G and -H. Three of them (HLA-E, -F and -G) were shown to inhibit NK activity. On the contrary, the fourth one, HLA-H, named HFE after it was found to be mutated in patients suffering from inherited hemochromatosis, has been shown to be involved only in the regulation of iron uptake. We tested the capacity of HFE to affect (enhance or reduce) specifically the NK activity contained in non-manipulated fresh human PBMCs. We showed that HFE expression by target cells does not affect their killing by the NK-like activity contained in PBMCs. Moreover, using fluorescent HFE tetramers, we could confirm that blood NK cells as well as blood {gamma}{delta} T cells do not bind HFE. Altogether, our data indicate that HFE does not affect the NK activity contained in the PBMCs.

Keywords: HFE; iron metabolism; NK cells; non-classical MHC class I; tetramer.
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