Skip Navigation



International Immunology Advance Access published online on November 29, 2004
International Immunology, doi:10.1093/intimm/dxh187
© 2004 by The Japanese Society for Immunology
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
17/1/65    most recent
dxh187v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Okoshi, Y.
Right arrow Articles by Shibuya, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Okoshi, Y.
Right arrow Articles by Shibuya, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received August 10, 2004
Accepted October 19, 2004

Article

Requirement of the tyrosines at residues 258 and 270 of MAIR-I in inhibitory effect on degranulation from basophilic leukemia RBL-2H3

Yasushi Okoshi 1, Satoko Tahara-Hanaoka 2, Chigusa Nakahashi 2, Shin-ichiro Honda 2, Akitomo Miyamoto 2, Hiroshi Kojima 3, Toshiro Nagasawa 3, Kazuko Shibuya 2, and Akira Shibuya 2*

1 Department of Immunology, Institute of Basic Medical Sciences, University of Tsukuba, Japan; Department of Clinical and Experimental Hematology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Japan
2 Department of Immunology, Institute of Basic Medical Sciences, University of Tsukuba, Japan
3 Department of Clinical and Experimental Hematology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Japan

* To whom correspondence should be addressed.
Akira Shibuya, E-mail: ashibuya{at}md.tsukuba.ac.jp


  Abstract

Mast cells and basophils express the high affinity receptor for IgE (Fc{varepsilon}RI) and play a central role for IgE-associated immediate hypersensitivity reactions and allergic disorders. Cross-linking of Fc{varepsilon}RI-bound IgE with multivalent antigen initiates the activation of mast cells and basophils, resulting in the degranulation from these cells. We have recently identified a novel inhibitory receptor, myeloid-associated immunoglobulin-like receptor (MAIR)-I, which is expressed on mast cells as well as other myeloid cell lineages. Co-ligation of Fc{varepsilon}RI and MAIR-I inhibits IgE-mediated degranulation from mast cells. However, MAIR-I-mediated signaling pathways involved in the inhibition remain undetermined. Here, we demonstrate that the transfectant of rat basophil leukemia RBL-2H3 expressing wild-type MAIR-I is tyrosine phosphorylated and recruits SHP-1 and SHIP upon cross-linking of MAIR-I. By using RBL-2H3 transfectants expressing variable mutant MAIR-I at Y233, Y258, Y270 and/or Y299, we further demonstrate that both Y258 and Y270, but not Y233 and Y299, were phosphorylated and were essentially required for inhibition of IgE-mediated degranulation from the RBL-2H3 transfectant.

Keywords: Fc{varepsilon}RI; inhibitory signal; ITIM; mast cell.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
K. Izawa, J. Kitaura, Y. Yamanishi, T. Matsuoka, A. Kaitani, M. Sugiuchi, M. Takahashi, A. Maehara, Y. Enomoto, T. Oki, et al.
An Activating and Inhibitory Signal from an Inhibitory Receptor LMIR3/CLM-1: LMIR3 Augments Lipopolysaccharide Response through Association with FcR{gamma} in Mast Cells
J. Immunol., July 15, 2009; 183(2): 925 - 936.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Nakata, T. Yoshimaru, Y. Suzuki, T. Inoue, C. Ra, H. Yakura, and K. Mizuno
Positive and Negative Regulation of High Affinity IgE Receptor Signaling by Src Homology Region 2 Domain-Containing Phosphatase 1
J. Immunol., October 15, 2008; 181(8): 5414 - 5424.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
I. Can, S. Tahara-Hanaoka, K. Hitomi, T. Nakano, C. Nakahashi-Oda, N. Kurita, S.-i. Honda, K. Shibuya, and A. Shibuya
Caspase-Independent Cell Death by CD300LF (MAIR-V), an Inhibitory Immunoglobulin-Like Receptor on Myeloid Cells
J. Immunol., January 1, 2008; 180(1): 207 - 213.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
T. Ozawa, K. Nakata, K. Mizuno, and H. Yakura
Negative autoregulation of Src homology region 2-domain-containing phosphatase-1 in rat basophilic leukemia-2H3 cells
Int. Immunol., September 1, 2007; 19(9): 1049 - 1061.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Nakahashi, S. Tahara-Hanaoka, N. Totsuka, Y. Okoshi, T. Takai, N. Ohkohchi, S.-i. Honda, K. Shibuya, and A. Shibuya
Dual Assemblies of an Activating Immune Receptor, MAIR-II, with ITAM-Bearing Adapters DAP12 and FcR{gamma} Chain on Peritoneal Macrophages
J. Immunol., January 15, 2007; 178(2): 765 - 770.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.