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International Immunology Advance Access published online on November 22, 2004
International Immunology, doi:10.1093/intimm/dxh181
© 2004 by The Japanese Society for Immunology
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Received June 25, 2004
Accepted September 30, 2004

Article

Phenotypical and functional alterations in the mucosal immune system of CD45 exon 9 KO mice

María C. López 1* and Nick Holmes 2

1 Department of Pathology, University of Cambridge, Cambridge CB2 1QP, UK; Present address: Center for Immunology & Microbial Disease, Albany Medical College, MC-151, 47 New Scotland Avenue, Albany, NY 12208-3479, USA
2 Department of Pathology, University of Cambridge, Cambridge CB2 1QP, UK

* To whom correspondence should be addressed.
María C. López, E-mail: lopezma{at}mail.amc.edu


  Abstract

The protein tyrosine phosphatase CD45 has been shown to be an important regulator of antigen-receptor signaling in both T and B lymphocytes. Lymphocyte populations within the gut mucosa are phenotypically and ontogenetically different from those generally found in secondary lymphoid tissue. In T lymphopenic mice, extrathymic T cell development takes place within the gut. Here we report the characterization of T and B cell populations in the distinct compartments of the gut mucosa and gut-associated lymphoid tissue of CD45-null mice. These data suggest that CD45 is required for the development of the specialized T cell populations within the gut environment as has been previously shown for thymic development. We demonstrate that within the large intestine intraepithelial compartment {alpha}{beta}-TCR+ CD4+ T cells are selectively retained by CD45KO mice. T cells and NK cells within the intraepithelium and the gut mucosa associated lymphoid tissue of CD45KO mice frequently possess an activated phenotype, differentiated to produce typical TH1 and TH2 cytokines. These data demonstrate that local environmental differences within the gut can, at least in part, overcome the requirement for CD45 during activation of T cells.

Keywords: intestinal intraepithelial lymphocytes; lamina propria lymphocytes; Peyer's patches; NK cells.
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