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International Immunology Advance Access published online on November 4, 2004
International Immunology, doi:10.1093/intimm/dxh179
© 2004 by The Japanese Society for Immunology
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Received November 24, 2003
Accepted September 28, 2004

Article

Negative regulatory effect of histamine in DNFB-induced contact hypersensitivity

Edina Garaczi 1*, Márta Széll 2, Tamás Jánossy 3, Andrea Koreck 1, Andor Pivarcsi 4, Edit Buzás 5, Zoltán Pos 5, András Falus 5, Attila Dobozy 4, and Lajos Kemény 4

1 Department of Dermatology and Allergology, University of Szeged, Budapest, Hungary
2 Dermatological Research Group of the Hungarian Academy of Sciences at the Department of Dermatology and Allergology, University of Szeged, Budapest, Hungary
3 Institute of Surgical Research, University of Szeged, Budapest, Hungary
4 Department of Dermatology and Allergology, University of Szeged, Budapest, Hungary; Dermatological Research Group of the Hungarian Academy of Sciences at the Department of Dermatology and Allergology, University of Szeged, Budapest, Hungary
5 Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary

* To whom correspondence should be addressed.
Edina Garaczi, E-mail: egaraczi{at}yahoo.com


  Abstract

Histamine plays an important role in the regulation of various immunological functions. To evaluate the role of histamine in contact hypersensitivity, contact dermatitis was induced with dinitrofluorobenzene (DNFB) in histidine decarboxylase knockout (HDC-/-) histamine-deficient and wild-type mice. The DNFB-induced increase of the ear thickness was significantly higher in HDC-/- mice than in wild-type mice. Using flow cytometry, significantly lower percentages of CD4+ Th and CD8+ Tc cells, and significantly higher percentages of CD45R+ B cells were observed in the regional lymph nodes in HDC-/- mice than in wild-type mice. In the ear specimens of both groups, the majority of the infiltrating cells were neutrophils and macrophages at 24 and 48 h after challenge. Using immunohistochemistry, we observed significantly more CD45+ leukocytes in HDC-/- mice than in wild-type mice. The expression of Th1 (IL-2, IFN-{gamma}, TNF-{alpha}) and Th2 (IL-4) mRNAs was examined by quantitative real time RT-PCR in the ear samples. The levels of Th1 cytokine mRNAs both at 24 and 48 h after challenge and IL-4 mRNA at 48 h showed a significantly higher increase in HDC-/- mice than in wild-type mice. These results suggest that histamine plays a negative immunoregulatory role in DNFB-induced contact hypersensitivity.

Keywords: delayed type hypersensitivity; histidine decarboxylase; immunoregulation.
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