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International Immunology Advance Access published online on September 27, 2004
International Immunology, doi:10.1093/intimm/dxh168
© 2004 by The Japanese Society for Immunology
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Received March 1, 2004
Accepted August 30, 2004

Article

Functional T cells in primary immune response to histoplasmosis

Jr-Shiuan Lin 1 and Betty A. Wu-Hsieh 1*

1 Graduate Institute of Immunology, National Taiwan University College of Medicine, Taipei, Taiwan, Republic of China

* To whom correspondence should be addressed. E-mail: wuhsiehb{at}ha.mc.ntu.edu.tw.


  Abstract

Functional T cells are critical to host defense against infection. It has been reported that functional T cells as determined by their cytokine production represent antigen-specific T cells in infectious disease models. In this study, we enumerated Histoplasma-specific interferon {gamma}-producing cells in bulk splenocyte culture and showed that infection with Histoplasma capsulatum, an intracellular pathogen of the macrophage, activated both CD4 and CD8 T cells. The magnitude of CD8 T cell response was lower than CD4 T cell, but the expansion and contraction of both cell types followed the same kinetics. Over 90% of interferon {gamma}-producing CD4 T cells and >85% of CD8 T cells expressed CD44hi phenotype. The strong correlation between interferon {gamma} production and CD44hi expression was observed not only at the peak of response but also throughout the course of infection. Moreover, a broad spectrum of V{beta} populations responded to systemic as well as pulmonary infections, suggesting no obvious T cell receptor bias in primary immune response to histoplasmosis. While each V{beta} population contributed to interferon {gamma} production, several specific V{beta} populations made up higher percentages of interferon {gamma}-producing cells. Our study laid the groundwork for further investigations in immune response to histoplasmosis.

Keywords: CD44; histoplasmosis; IFN{gamma}-producing cells; specific T cell response; V{beta} repertoire.
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