International Immunology Advance Access published online on August 31, 2004
International Immunology, doi:10.1093/intimm/dxh150
© 2004 by The Japanese Society for Immunology
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1 Department of Dermatology, Keio University School of Medicine, Tokyo 160-8582, Japan; Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo 160-8582, Japan
* To whom correspondence should be addressed. E-mail: koyasu{at}sc.itc.keio.ac.jp.
To examine the mechanism of B cell tolerance against natural peripheral self-antigen, we generated transgenic mice expressing IgM specific for desmoglein 3 (Dsg3) from AK7 monoclonal antibody which itself does not induce blisters. Dsg3 is mainly expressed on stratified squamous epithelium and is the target antigen of an autoimmune bullous disease, pemphigus vulgaris. Transgenic B cells reactive to Dsg3 were observed in the spleen and lymph node. Although these B cells are autoreactive, they did not develop into B1 B cells. These B cells were functionally competent and anti-Dsg3 IgM was detected in the serum and on the keratinocyte cell surface. These results indicate that auto-reactive B cells against peripheral antigen (Dsg3) are able to develop in the presence of Dsg3 but are ignored by the immune system.
Accepted July 25, 2004
Article
Auto-reactive B cells against peripheral antigen, desmoglein 3, escape from tolerance mechanism
2 Department of Dermatology, Keio University School of Medicine, Tokyo 160-8582, Japan
3 Department of Dermatology, Keio University School of Medicine, Tokyo 160-8582, Japan; Department of Dentistry and Oral Surgery, Keio University School of Medicine, Tokyo 160-8582, Japan
4 Laboratory Animal Center, Keio University School of Medicine, Tokyo 160-8582, Japan
5 Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo 160-8582, Japan; Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kawaguchi 332-0012, Japan
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