Skip Navigation



International Immunology Advance Access published online on June 21, 2004
International Immunology, doi:10.1093/intimm/dxh112
© 2004 by The Japanese Society for Immunology
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary data
Right arrow All Versions of this Article:
16/8/1109    most recent
dxh112v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Lee, M. S.
Right arrow Articles by McCune, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, M. S.
Right arrow Articles by McCune, J. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received February 12, 2004
Accepted May 17, 2004

Article

Gene expression profiles during human CD4+ T cell differentiation

Myeong Sup Lee 1, Kristina Hanspers 2, Christopher S. Barker 3, Abner P. Korn 4, Joseph M. McCune 1*

1 Gladstone Institute of Virology and Immunology, University of California at San Francisco, San Francisco, CA 94141, USA; Department of Medicine, University of California at San Francisco, San Francisco, CA 94143, USA; Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, CA 94143, USA
2 Genomics Core Laboratory, Gladstone Institute of Cardiovascular Disease, University of California at San Francisco, San Francisco, CA 94141, USA
3 Genomics Core Laboratory, Gladstone Institute of Cardiovascular Disease, University of California at San Francisco, San Francisco, CA 94141, USA; General Clinical Research Center, Core Genomics Laboratory, San Francisco General Hospital, San Francisco, CA 94110, USA
4 Department of Obstetrics, Gynecology, and Reproductive Biology, San Francisco General Hospital, San Francisco, CA 94110, USA

* To whom correspondence should be addressed. E-mail: mmccune{at}gladstone.ucsf.edu.


  Abstract

To develop a comprehensive catalogue of phenotypic and functional parameters of human CD4+ T cell differentiation stages, we have performed microarray gene expression profiling on subpopulations of human thymocytes and circulating naive CD4+ T cells, including CD3-CD4+CD8- intrathymic T progenitor cells, CD3intCD4+CD8+ ‘double positive’ thymocytes, CD3highCD4+CD8- ‘single positive’ thymocytes, CD3+CD4+CD8- CD45RA+CD62L+ naive T cells from cord blood and CD3+CD4+CD8- CD45RA+CD62L+ naive T cells from adult blood. These subpopulations were sort-purified to >98% purity and their expressed RNAs were analyzed on Affymetrix Human Genome U133 arrays. Comparison of gene expression signals between these subpopulations and with early passage fetal thymic stromal cultures identify: (i) transcripts that are preferentially expressed in human CD4+ T cell subpopulations and not in thymic stromal cells; (ii) major shifts in gene expression as progenitor T cells mature into progeny; (iii) preferential expression of transcripts at the progenitor cell stage with plausible relevance to the regulation of expansion and differentiation of these cells; and (iv) preferential expression of potential markers of recent thymic emigrants in naive-phenotype CD4+ T cells from cord blood. Further evaluation of these findings may lead to a better definition of human thymopoiesis as well as to improved approaches to monitor and to augment the function of this important organ of T cell production.

Keywords: gene expression; microarray; naive CD4+ T cell; recent thymic emigrants; thymocytes
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
C. C. Tydell, E.-S. David-Fung, J. E. Moore, L. Rowen, T. Taghon, and E. V. Rothenberg
Molecular Dissection of Prethymic Progenitor Entry into the T Lymphocyte Developmental Pathway
J. Immunol., July 1, 2007; 179(1): 421 - 438.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. D. Hyrcza, C. Kovacs, M. Loutfy, R. Halpenny, L. Heisler, S. Yang, O. Wilkins, M. Ostrowski, and S. D. Der
Distinct Transcriptional Profiles in Ex Vivo CD4+ and CD8+ T Cells Are Established Early in Human Immunodeficiency Virus Type 1 Infection and Are Characterized by a Chronic Interferon Response as Well as Extensive Transcriptional Changes in CD8+ T Cells
J. Virol., April 1, 2007; 81(7): 3477 - 3486.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. J. Munoz, D. Alfaro, J. Garcia-Ceca, L. M. Alonso-C, E. Jimenez, and A. Zapata
Thymic Alterations in EphA4-Deficient Mice
J. Immunol., July 15, 2006; 177(2): 804 - 813.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.