International Immunology, Vol 9, 1011-1019, Copyright © 1997 by Oxford University Press
H Kawamoto, K Ohmura and Y Katsura
We established an experimental system in vitro to examine the developmental
capacity of individual hematopoietic progenitors to generate T, B and
myeloid (M) cells. By using this system we analyzed the process of lineage
commitment of hematopoietic progenitors in murine fetal liver (FL). It is
known that small numbers of B and M cells, in addition to T cells, are
generated in a co-culture of hematopoietic progenitors and a
deoxyguanosine-treated fetal thymus (FT) lobe. We tried to enhance the
growth of B and M cells by the addition of IL-7, IL-3 and stem cell factor
into the co-culture. This cytokine-supplemented FT organ culture was used
to examine the developmental capacity of individual hematopoietic
progenitors in FL. Single cells of lineage marker (Lin)-c-kit+Sca-1+
(Sca-1+) and Lin-c- kit+Sca-1-(Sca-1-) populations from the FL harvested at
day 12 of gestation were cultured for 10 days, and the phenotypes of cells
generated in each lobe were analyzed with a flow cytometer. All progenitors
in the Sca-1- population were shown to be committed to generate only T, B
or M cells. On the other hand, multipotent progenitors, which are capable
of generating T, B and M cells, as well as unipotent progenitors committed
to the T, B or M lineage were found in the Sca-1+ population. Bipotent
progenitors generating M and T cells and those generating M and B cells
were also found in the Sca-1+ population, which probably represent
progenitors in the process of commitment. However, no bipotent progenitors
generating T and B cells were detected.
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Direct evidence for the commitment of hematopoietic stem cells to T, B and myeloid lineages in murine fetal liver
Department of Immunology, Chest Disease Research Institute, Kyoto University, Japan.
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|
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|
|
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|
|
|
|
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|
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|
|
|
|
|
|
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|
|
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|
|
|
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|
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