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International Immunology Advance Access originally published online on June 5, 2009
International Immunology 2009 21(7):757-767; doi:10.1093/intimm/dxp044
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© The Japanese Society for Immunology. 2009. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Synthetic methylated CpG ODNs are potent in vivo adjuvants when delivered in liposomal nanoparticles

Ghania Chikh1,*, Susan D. de Jong2,*, Laura Sekirov1, Sameersingh G. Raney1, Mikameh Kazem1,2, Kaley D. Wilson2, Pieter R. Cullis2, Jan P. Dutz3 and Ying K. Tam1

1 Tekmira Pharmaceuticals Corporation, Burnaby, British Columbia, Canada
2 Department of Biochemistry and Molecular Biology
3 Department of Dermatology and Skin Science and Children and Family Research Institute, University of British Columbia, Vancouver, British Columbia, Canada

Correspondence to: K. D. Wilson, Centre for Drug Research and Development, University of British Columbia, Suite 364-2259 Lower Mall, Vancouver, British Columbia, Canada V6T 1Z4; E-mail: kwilson{at}cdrd.ca

Although it is well documented that the immunological activity of cytosine–guanine (CpG) motifs is abrogated by 5' methylation of the cytosine residue, encapsulation within stabilized lipid nanoparticles endows these methylated cytosine–guanine- (mCpG-) containing oligonucleotides (ODNs) with potent immunostimulatory activity in murine animal models. Surprisingly, not only do liposomal nanoparticulate (LN) mCpG ODN possess immunostimulatory activity, their potency is found to be equivalent and often greater than the equivalent unmethylated form, as judged by a number of ex vivo innate and adaptive immune parameters and anti-tumor efficacy in murine models. Preliminary data indicate that both methylated and unmethylated CpG ODN act through a common receptor signaling pathway, specifically via toll-like receptor (TLR) 9, based on observations of up-regulated TLR9 expression, induction of nitric oxide and dependence on endosomal maturation. This is confirmed in TLR9 knockout animals which show no immunostimulatory activity following treatment with LN-mCpG ODN. These data, therefore, indicate that the mCpG DNA is fully competent to interact with TLR9 to initiate potent immune responses. Furthermore, this work implicates an as yet unidentified mechanism upstream of TLR9 which regulates the relative activities of free methylated versus unmethylated CpG ODN that is effectively bypassed by particulate delivery of CpG ODN.

Keywords: cell activation, cytotoxicity, immunotherapy, rodent, TLR9

* These authors contributed equally to this study.

Transmitting editor: L. Moretta

Received 2 August 2008, accepted 14 April 2009.


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