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International Immunology Advance Access originally published online on July 21, 2008
International Immunology 2008 20(9):1211-1218; doi:10.1093/intimm/dxn079
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© The Japanese Society for Immunology. 2008. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Manipulation of immune system via immortal bone marrow stem cells

Christiane Ruedl, Hanif Javanmard Khameneh and Klaus Karjalainen

School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore

Correspondence to: Christiane Ruedl; E-mail: ruedl{at}ntu.edu.sg

Extensive amplification of hematopoietic stem cells (HSCs) and their multipotent primitive progenitors (MPPs) in culture would greatly benefit not only clinical transplantation but also provide a potential tool to manipulate all cellular lineages derived from these cells for gene therapy and experimental purposes. Here, we demonstrate that mouse bone marrow cultures containing cells engineered to over-express NUP98–HOXB4 fusion protein support self-renewal of physiologically normal HSC and MPP for several weeks leading practically to their unlimited expansion. This allows time consuming and cumulative in vitro experimental manipulations without sacrificing their ability to differentiate in vivo or in vitro to any hematopoietic lineage.

Keywords: hematopoietic stem cells, manipulation of immune system, NUP98-HOXB4

Transmitting editor: M. Reth

Received 25 April 2008, accepted 17 June 2008.


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