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International Immunology Advance Access originally published online on November 13, 2007
International Immunology 2008 20(1):81-88; doi:10.1093/intimm/dxm122
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© The Japanese Society for Immunology. 2007. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Priming of CD4+ T cells with porin of Shigella dysenteriae activates the cells toward type 1 polarization

Amlan Biswas, Pallavi Banerjee and Tapas Biswas

Division of Immunology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme XM, Kolkata 700 010, West Bengal, India

Correspondence to: T. Biswas; E-mail: shigellaporin{at}yahoo.com

Macrophages treated with porin of Shigella dysenteriae were potent stimulators of naive Th cells since the porin-pulsed macrophages strongly proliferated the T cells and up-regulated the activation molecules CD69 and CD25 on CD4+ T cells in allogeneic mixed leukocyte reaction. Immunization of C57BL/6 mice with porin selectively induced the intracellular expression and release of IFN-{gamma} and had no effect on IL-4 expression. In parallel to the predominant release of the Th1 cytokine IFN-{gamma}, up-regulation of CCR5 on the immune CD4+ T cells and messenger ribonucleic acid (mRNA) expression of the T cell chemokines macrophage inflammatory protein-1{alpha} and regulated on activation normal T cell expressed and secreted showed Th1 bias. The porin-primed CD4+ T cells expressed the mRNA for T-box expressed in T cells, a Th1-specific transcription factor confirming the adjuvant-induced transition of T cells to polarized effector Th1 cells. The immune CD4+ T cells proliferated and released IL-2 and IFN-{gamma} profoundly in response to re-challenge with porin-pulsed macrophages but not to BSA-pulsed macrophages in vitro, which demonstrated the presence of porin-specific CD4+ T cells of Th1 phenotype. The study highlights that porin has the capacity of an adjuvant to unfold and maintain the T cell function and thereby to activate adaptive immunity.

Keywords: chemokines, IFN-{gamma}, porin, Th cells, type 1 polarization

Transmitting editor: A. Falus

Received 8 August 2007, accepted 17 October 2007.


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