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International Immunology Advance Access originally published online on November 20, 2007
International Immunology 2008 20(1):105-116; doi:10.1093/intimm/dxm125
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© The Author 2007. Published by Oxford University Press on behalf of The Japanese Society for Immunology. All rights reserved.
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press and The Japanese Society for Immunology are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Many human peripheral VH5-expressing IgM+ B cells display a unique heavy-chain rearrangement

Annick Lim1,2, Brigitte Lemercier1,2, Xavier Wertz3, Sarah Lesjean Pottier4,2, François Huetz5 and Philippe Kourilsky1,3

1 Unité du développement des lymphocytes
2 INSERM U668, Institut Pasteur, Paris, France
3 Collège de France, Paris, France
4 Unité des Cytokines et Développement lymphoïde, INSERM U668
5 Unité d'Immunité cellulaire antivirale, Institut Pasteur, Paris, France

Correspondence to: A. Lim, Unité d'Immunité Anti-virale, Biothérapie et Vaccins, Institut Pasteur, 25 Rue du Dr Roux, 75724 Paris CEDEX 15, France. E-mail: alim{at}pasteur.fr

The immunoscope methodology has proven useful to analyze T-cell repertoires in mice and humans. We adapted it to the analysis of VH chains of human peripheral B cells by setting up a quantification of various VH and JH segments and the profiling of IgM-, IgG-, IgA- and IgE-expressing B cells. We then tested the hypothesis that the human B-cell and T-cell repertoires have a similar diversity of VH and V-beta rearrangements. We studied in more detail the VH5 family because it is not abundantly used, which facilitated the analysis. The data showed that the number of distinct VH5 rearrangements in all samples studied is close to the number of cells in the sample. This contrasts with T cells in which we previously showed that distinct V-beta rearrangements amount to a few percent of the number of T cells because each V-beta chain is on the average paired with ~25 alpha chains. Thus, in the VH5 family, the light chains add little quantitative diversity to that produced by the heavy chain alone. Whether this feature can be generalized to other VH chains is discussed.

Keywords: B lymphocytes, clone size, human, repertoire, somatic mutation diversity

Transmitting editor: E. Vivier

Received 15 March 2007, accepted 19 October 2007.


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