International Immunology Advance Access originally published online on March 31, 2006
International Immunology 2006 18(6):857-869; doi:10.1093/intimm/dxl022
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Expression of human DEC-205 (CD205) multilectin receptor on leukocytes
1 Mater Medical Research Institute, Dendritic Cell Laboratory, Aubigny Place, Raymond Terrace, Queensland 4101, Australia
2 Cooperative Research Centre for Chronic Inflammatory Diseases, Institute for Molecular Biosciences, University of Queensland, St Lucia, Queensland 4067, Australia
3 Queensland Institute of Medical Research, Leukaemia Foundation Laboratory, Queensland 4006, Australia
4 School of Medicine, University of Queensland, Herston Road, Queensland 4006, Australia
Correspondence to: M. Kato; E-mail: mkato{at}mmri.mater.org.au
DEC-205 (CD205) belongs to the macrophage mannose receptor family of C-type lectin endocytic receptors and behaves as an antigen uptake/processing receptor for dendritic cells (DC). To investigate DEC-205 tissue distribution in human leukocytes, we generated a series of anti-human DEC-205 monoclonal antibodies (MMRI-5, 6 and 7), which recognized epitopes within the C-type lectin-like domains 1 and 2, and the MMRI-7 immunoprecipitated a single 
200 kDa band, identified as DEC-205 by mass spectrometry. MMRI-7 and another DEC-205 mAb (MG38), which recognized the epitope within the DEC-205 cysteine-rich and fibronectin type II domain, were used to examine DEC-205 expression by human leukocytes. Unlike mouse DEC-205, which is reported to have predominant expression on DC, human DEC-205 was detected by flow cytometry at relatively high levels on myeloid blood DC and monocytes, at moderate levels on B lymphocytes and at low levels on NK cells, plasmacytoid blood DC and T lymphocytes. MMRI-7 F(ab')2 also labeled monocytes, B lymphocytes and NK cells similarly excluding reactivity due to non-specific binding of the mAb to Fc
R. Tonsil mononuclear cells showed a similar distribution of DEC-205 staining on the leukocytes. DEC-205-specific semiquantitative immunoprecipitation/western blot and quantitative reverse transcriptase-PCR analysis established that these leukocyte populations expressed DEC-205 protein and the cognate mRNA. Thus, human DEC-205 is expressed on more leukocyte populations than that were previously assumed based on mouse DEC-205 tissue localization studies. The broader DEC-205 tissue expression in man is relevant to clinical DC targeting strategies and DEC-205 functional studies.
Keywords: cell surface molecules, C-type lectins, dendritic cells, immunotherapy
Transmitting editor: M. Miyasaki
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