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International Immunology Advance Access originally published online on March 28, 2006
International Immunology 2006 18(5):767-773; doi:10.1093/intimm/dxl012
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© The Japanese Society for Immunology. 2006. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

A redundant role for PKC-{varepsilon} in mast cell signaling and effector function

Eva Lessmann1, Michael Leitges2,3, and Michael Huber1

1 Department of Molecular Immunology, Institute for Biology III, University of Freiburg and Max Planck Institute for Immunobiology, Stübeweg 51, 79108 Freiburg, Germany
2 Department of Medicine, Medical School Hannover, 30625 Hannover, Germany
3 Max Planck Institute for Experimental Endocrinology, 30625 Hannover, Germany

Correspondence to: Michael Huber; E-mail: huberm{at}immunbio.mpg.de

Protein kinase (PK) C-{varepsilon} is strongly expressed in mast cells (MCs) and activated in response to antigen-mediated high-affinity receptor for IgE (Fc{varepsilon}R1) engagement. A critical role of PKC-{varepsilon} in antigen-triggered activation of various signaling pathways was observed in basophilic leukemia cells. To study the function of PKC-{varepsilon} in MCs differentiated in vitro from murine bone marrow, we used our established PKC-{varepsilon} null mice. Unexpectedly, we did not reveal any difference in antigen-induced activation of many central signaling molecules (PKB, mitogen-activated protein kinase, p38, Jun-N-terminal kinase, phospholipase C-{gamma}1, Bruton's tyrosine kinase, PKD, Fos and PKC-{delta}) in time-course as well as dose-response studies between PKC-{varepsilon}-deficient and wild-type MCs. In correlation, antigen-triggered degranulation, release of arachidonic acid and secretion of IL-6 were unaltered by the loss of PKC-{varepsilon}. Furthermore, stimulation of MCs via different receptor systems [Steel factor receptor (c-kit) and toll-like receptor 4] did not lead to differences in the measured responses between both cell types. These results strongly suggest that PKC-{varepsilon} plays a redundant role in MCs stimulated by antigen as well as other well-known MC stimuli.

Keywords: degranulation, Fc{varepsilon}R1, IL-6, signal transduction, TLR4

Transmitting editor: I. Pecht


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