International Immunology Advance Access originally published online on January 13, 2006
International Immunology 2006 18(3):415-423; doi:10.1093/intimm/dxh382
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
The proliferative response of CD4 T cells to steady-state CD8+ dendritic cells is restricted by post-activation death
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
Correspondence to: K. Shortman; E-mail: shortman{at}wehi.edu.au
CD8+ splenic dendritic cells (DCs) from steady-state mice are less effective than the CD8– DC subset in their capacity to stimulate CD4 T cell proliferation in culture. However, we found that the two DC subtypes were equally potent at activating CD4 T cells, based on up-regulation of CD69 and CD25 expression. Also, we found no difference in the rate of T cell death prior to entry into the first division. We then tracked carboxyfluorescein diacetate succinimidyl ester-labeled T cells and employed a quantitative model to assess in detail the CD4 T cell expansion process in response to stimulation with CD8+ or with CD8– DCs. The time required for most T cells to replicate their DNA prior to the first division was similar in both DC cultures. However, progression of the CD4 T cell population through subsequent divisions was reduced in CD8+ DCs compared with CD8– DC culture. This was associated with an increased loss of viable T cells at each division. Post-activation, division-associated T cell death is therefore a major factor in the reduced response of CD4 T cells to CD8+ DCs.
Keywords: dendritic cells, modelling, T cells, T cell proliferation
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Huang, M. Parker, C. Xia, R. Peng, C. Wasserfall, T. Clarke, L. Wu, T. Chowdhry, M. Campbell-Thompson, J. Williams, et al. Rabbit Polyclonal Mouse Antithymocyte Globulin Administration Alters Dendritic Cell Profile and Function in NOD Mice to Suppress Diabetogenic Responses J. Immunol., April 15, 2009; 182(8): 4608 - 4615. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Marleau, K. L. Summers, and B. Singh Differential Contributions of APC Subsets to T Cell Activation in Nonobese Diabetic Mice J. Immunol., April 15, 2008; 180(8): 5235 - 5249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Galazka, A. Jurewicz, W. Orlowski, M. Stasiolek, C. F. Brosnan, C. S. Raine, and K. Selmaj EAE Tolerance Induction with Hsp70-Peptide Complexes Depends on H60 and NKG2D Activity J. Immunol., October 1, 2007; 179(7): 4503 - 4512. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. S. Kim, S. H. Yang, H. G. Kang, E. Y. Seong, S. H. Lee, W. Gao, J. Kenny, X. X. Zheng, and T. B. Strom Distinctive role of donor strain immature dendritic cells in the creation of allograft tolerance Int. Immunol., December 1, 2006; 18(12): 1771 - 1777. [Abstract] [Full Text] [PDF]
|
|