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International Immunology Advance Access originally published online on August 15, 2005
International Immunology 2005 17(9):1257-1268; doi:10.1093/intimm/dxh302
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© The Japanese Society for Immunology. 2005. All rights reserved. For permissions, please e-mail: journals.permissions@oupjournals.org

Expression of the Ian family of putative GTPases during T cell development and description of an Ian with three sets of GTP/GDP-binding motifs

Carine Dion1, Christine Carter1, Lucy Hepburn1, W. John Coadwell1, Geoff Morgan1, Margaret Graham1, Nicholas Pugh1, Graham Anderson2, Geoffrey W. Butcher1 and J. Ross Miller1

1 Babraham Institute, Cambridge CB2 4AT, UK
2 Department of Anatomy, Medical School, Birmingham University, Edgbaston, UK

Correspondence to: G. W. Butcher; E-mail: geoff.butcher{at}bbsrc.ac.uk

Reports suggest that two members of the novel immune-associated nucleotide (Ian) GTPase family, Ian1 and Ian5, play roles in T cell development. We performed real-time PCR analysis of the expression of Ian genes of the rat during T cell maturation, in macrophages and in cell lines. We found that all of the genes were expressed at relatively low levels at the early double-negative thymocyte stage but were expressed more strongly at later cell stages. Our study also revealed the fact that the previously reported Ian9, Ian10 and Ian11 genes are, instead, parts of a single gene for which we retain the name Ian9, potentially encoding a GTPase with a highly unusual triplicated structure. Antisera were developed against both Ian1 and Ian9. We established that Ian9 is produced as an ~75-kDa protein in both T cells and thymocytes. We observed that levels of both Ian1 and Ian9 proteins are profoundly reduced in T cells from lymphopenic rats as compared with wild-type rats. It was demonstrated that thymocytes and B cells from lymphopenic rats (Ian5 null) did not show enhanced sensitivity to {gamma}-irradiation-induced apoptosis.

Keywords: diabetes, GTPases, lymphopenia, rat, T lymphocytes

Transmitting editor: A. Cooke


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