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International Immunology Advance Access originally published online on October 11, 2005
International Immunology 2005 17(12):1553-1560; doi:10.1093/intimm/dxh333
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© The Japanese Society for Immunology. 2005. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

The positional effect of Eß on Vß genes of TCRß chain in the ordered rearrangement and allelic exclusion

Daisuke Suzuki1, Lili Wang1, Makoto Senoo2 and Sonoko Habu1

1 Department of Immunology, Tokai University School of Medicine, Bouseidai, Isehara, Kanagawa 259-1193, Japan
2 Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA

Correspondence to: S. Habu; E-mail: sonoko{at}is.icc.u-tokai.ac.jp

In the TCRß gene locus, the Vß, Dß and Jß gene segments are assembled in a tightly ordered manner. To investigate the positional effects of TCRß enhancer (Eß) on the recombination processes of the Vß genes, we utilized ßLD mice lacking 70% of the TCRß locus, leaving four Vß genes at the 5' side and, consequently, the Vß10 gene moves into the Eß regulatory region. In this mutant mouse, the Vß10 gene showed direct Vß-to-Dß and Vß-to-Jß recombination, although the Dß-to-Jß joining was still predominant. Interestingly, these two aberrant recombination processes were barely suppressed when ßLD mice were crossed with TCRß transgenic mice, whereas V(D)J recombination of the Vß10 gene was sufficiently suppressed. These results suggest that the positional effects of Eß on the Vß genes may enable the recombination potential to increase prior to Dß-to-Jß joining and that such aberrant recombination may be free from allelic suppression.

Keywords: allelic exclusion, gene rearrangement, T cell receptors

Transmitting editor: K. Okumura


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