International Immunology Advance Access originally published online on September 2, 2005
International Immunology 2005 17(10):1337-1346; doi:10.1093/intimm/dxh312
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
IL-4 is more effective than IL-13 for in vitro differentiation of dendritic cells from peripheral blood mononuclear cells
Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB T6G 2S2, Canada
Correspondence to: B. Agrawal; E-mail: bagrawal{at}ualberta.ca
Dendritic cells (DCs) are the most potent professional antigen-presenting cells which can activate T cells to induce the primary immune response. For clinical studies, DCs are often differentiated in vitro from peripheral blood mononuclear cells (PBMCs) through treatment with granulocyte macrophage colony-stimulating factor (GM-CSF) and IL-4. However, IL-13, a cytokine closely related to IL-4, has also been reported to induce differentiation equally or more efficiently when used with GM-CSF. For the present study, we compared the DC characteristics exhibited by iDCs and LPS-matured DCs differentiated from PBMCs using GM-CSF and IL-4 or IL-13. Physical characteristics examined include cellular morphology and surface phenotype. Functional traits investigated include FITC–dextran uptake, IL-10 and IL-12 production, allostimulation and cytokine production by stimulated T cells and antigen-specific T cell stimulation. Compared with IL-13-derived DCs, IL-4 treatment yielded more differentiated DCs, with extensive dendrites and higher expression of DC-SIGN, DEC-205, CD86 and HLA-DR. In addition, IL-4 DCs were more efficient at inducing allogeneic T cell proliferation and immature IL-4 DCs had higher endocytic activity at low FITC–dextran concentrations (1 µg ml–1). Although IL-13 was capable of generating DCs from PBMCs, it was not as effective as IL-4 in generating DC phenotype and functionality. Thus, the use of GM-CSF and IL-4 is the more efficient treatment for inducing DC differentiation from PBMCs.
Keywords: cytokines, dendritic cells, immune response, T lymphocytes
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  E. den Dekker, S. Grefte, T. Huijs, G. B. ten Dam, E. M. M. Versteeg, L. C. J. van den Berk, B. A. Bladergroen, T. H. van Kuppevelt, C. G. Figdor, and R. Torensma Monocyte Cell Surface Glycosaminoglycans Positively Modulate IL-4-Induced Differentiation toward Dendritic Cells J. Immunol., March 15, 2008; 180(6): 3680 - 3688. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Li, D. K. Krishnadas, R. Kumar, D. L. J. Tyrrell, and B. Agrawal Priming and stimulation of hepatitis C virus-specific CD4+ and CD8+ T cells against HCV antigens NS4, NS5a or NS5b from HCV-naive individuals: implications for prophylactic vaccine Int. Immunol., January 1, 2008; 20(1): 89 - 104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Ahn, D. K. Krishnadas, and B. Agrawal Dendritic cells partially abrogate the regulatory activity of CD4+CD25+ T cells present in the human peripheral blood Int. Immunol., March 1, 2007; 19(3): 227 - 237. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Webb, Y. Cai, K. I. Matthaei, and P. S. Foster Comparative Roles of IL-4, IL-13, and IL-4R{alpha} in Dendritic Cell Maturation and CD4+ Th2 Cell Function J. Immunol., January 1, 2007; 178(1): 219 - 227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Grolleau-Julius, M. R. Garg, R. Mo, L. L. Stoolman, and R. L. Yung Effect of Aging on Bone Marrow-Derived Murine CD11c+CD4-CD8{alpha}- Dendritic Cell Function. J. Gerontol. A Biol. Sci. Med. Sci., October 1, 2006; 61(10): 1039 - 1047. [Abstract] [Full Text] [PDF]
|
|