Skip Navigation


International Immunology Advance Access originally published online on September 27, 2004
International Immunology 2004 16(11):1663-1673; doi:10.1093/intimm/dxh168
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
16/11/1663    most recent
dxh168v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Lin, J.-S.
Right arrow Articles by Wu-Hsieh, B. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lin, J.-S.
Right arrow Articles by Wu-Hsieh, B. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


© 2004 The Japanese Society for Immunology

Functional T cells in primary immune response to histoplasmosis

Jr-Shiuan Lin and Betty A. Wu-Hsieh

Graduate Institute of Immunology, National Taiwan University College of Medicine, Taipei, Taiwan, Republic of China

Correspondence to: B. A. Wu-Hsieh; E-mail: wuhsiehb{at}ha.mc.ntu.edu.tw

Functional T cells are critical to host defense against infection. It has been reported that functional T cells as determined by their cytokine production represent antigen-specific T cells in infectious disease models. In this study, we enumerated Histoplasma-specific interferon {gamma}-producing cells in bulk splenocyte culture and showed that infection with Histoplasma capsulatum, an intracellular pathogen of the macrophage, activated both CD4 and CD8 T cells. The magnitude of CD8 T cell response was lower than CD4 T cell, but the expansion and contraction of both cell types followed the same kinetics. Over 90% of interferon {gamma}-producing CD4 T cells and >85% of CD8 T cells expressed CD44hi phenotype. The strong correlation between interferon {gamma} production and CD44hi expression was observed not only at the peak of response but also throughout the course of infection. Moreover, a broad spectrum of Vß populations responded to systemic as well as pulmonary infections, suggesting no obvious T cell receptor bias in primary immune response to histoplasmosis. While each Vß population contributed to interferon {gamma} production, several specific Vß populations made up higher percentages of interferon {gamma}-producing cells. Our study laid the groundwork for further investigations in immune response to histoplasmosis.

Keywords: CD44, histoplasmosis, IFN{gamma}-producing cells, specific T cell response, Vß repertoire

Transmitting editor: S. H. E. Kaufmann


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Eukaryot CellHome page
L. Shi, P. C. Albuquerque, E. Lazar-Molnar, X. Wang, L. Santambrogio, A. Gacser, and J. D. Nosanchuk
A Monoclonal Antibody to Histoplasma capsulatum Alters the Intracellular Fate of the Fungus in Murine Macrophages
Eukaryot. Cell, July 1, 2008; 7(7): 1109 - 1117.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. Heninger, L. H. Hogan, J. Karman, S. Macvilay, B. Hill, J. P. Woods, and M. Sandor
Characterization of the Histoplasma capsulatum-Induced Granuloma.
J. Immunol., September 1, 2006; 177(5): 3303 - 3313.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J.-S. Lin, C.-W. Yang, D.-W. Wang, and B. A. Wu-Hsieh
Dendritic Cells Cross-Present Exogenous Fungal Antigens to Stimulate a Protective CD8 T Cell Response in Infection by Histoplasma capsulatum
J. Immunol., May 15, 2005; 174(10): 6282 - 6291.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.