Skip Navigation


International Immunology Advance Access originally published online on October 4, 2004
International Immunology 2004 16(11):1623-1631; doi:10.1093/intimm/dxh164
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
16/11/1623    most recent
dxh164v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (4)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Sun, L.
Right arrow Articles by Garzino-Demo, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sun, L.
Right arrow Articles by Garzino-Demo, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


© 2004 The Japanese Society for Immunology

Recall antigen activation induces prompt release of CCR5 ligands from PBMC: implication in memory responses and immunization

Lingling Sun, Sayed F. Abdelwahab1, George K. Lewis and Alfredo Garzino-Demo

Institute of Human Virology, University of Maryland Biotechnology Institute, 725 West Lombard Street, Room S613, Baltimore, MD 21201-1192, USA
1 Present address: Department of Microbiology and Immunology, El-minia Faculty of Medicine, El-minia University, El-minia 61111, Egypt

Correspondence to: A. Garzino-Demo; E-mail: garzinod{at}umbi.umd.edu

CCR5 ligands RANTES, macrophage inflammatory protein (MIP)-1{alpha} and MIP-1ß are potent and specific inhibitors of strains of human immunodeficiency virus (HIV) that use CCR5 as a receptor, which are the strains most involved in primary infection. Recently, we observed that release of CCR5 ligands is a consistent and reproducible parameter of response to antigen activation in studies using PBMC. In this study, we show that CCR5 ligands are released upon antigen [Fragment C of tetanus toxin (TTC)] stimulation in 81% (n = 16) of subjects tested, as detected by a standard ELISA in tissue culture supernatants of antigen-activated cells. In contrast, ELISA for other cytokines from the same supernatants revealed that IFN-{gamma} release could be detected only in 31% of subjects, IL-2 could be detected only in 12% of the subjects and IL-4 was not detectable in any of the subjects tested. Similarly, proliferative responses to TTC, as measured by a standard tritiated thymidine incorporation assay, were detectable in only 56% of the subjects. Similar observations have been reported in flow cytometric studies, and resonate with previous findings emphasizing the role of CCR5 in T cell responses. In addition, the levels of CCR5 ligands in supernatants from antigen-activated cells were sufficient to inhibit infection of R5 HIV. Thus, CCR5 ligands might play a role in controlling HIV in vivo. Taken together, these observations suggest that CCR5 ligands, and in particular MIP-1{alpha} and MIP-1ß, released in the course of memory responses may play a role in protecting CD4+ memory T cells from infection.

Keywords: chemokines, lymphoproliferation, MIP-1{alpha}, MIP-1ß, RANTES

Transmitting editor: G. Trinchieri


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
L. Sun, C. M. Finnegan, T. Kish-Catalone, R. Blumenthal, P. Garzino-Demo, G. M. La Terra Maggiore, S. Berrone, C. Kleinman, Z. Wu, S. Abdelwahab, et al.
Human {beta}-Defensins Suppress Human Immunodeficiency Virus Infection: Potential Role in Mucosal Protection
J. Virol., November 15, 2005; 79(22): 14318 - 14329.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.