Skip Navigation


International Immunology Advance Access originally published online on October 5, 2004
International Immunology 2004 16(11):1613-1622; doi:10.1093/intimm/dxh163
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
16/11/1613    most recent
dxh163v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by McKenzie, B. S.
Right arrow Articles by Lew, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McKenzie, B. S.
Right arrow Articles by Lew, A. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


© 2004 The Japanese Society for Immunology

Bypassing luminal barriers, delivery to a gut addressin by parenteral targeting elicits local IgA responses

Brent S. McKenzie1,5, Alexandra J. Corbett1, Susan Johnson1, Jamie L. Brady1, Jill Pleasance1, David R. Kramer2, Jefferey S. Boyle3, David C. Jackson4, Richard A. Strugnell4 and Andrew M. Lew1

1 The Walter and Eliza Hall Institute of Medical Research and Co-operative Research Centre for Vaccine Technology, 1G Royal Parade, Parkville 3050, Australia
2 Centre for Cellular and Molecular Biology, School of Biological and Chemical Sciences, Deakin University, Burwood 3125, Australia
3 CSL Ltd, 45 Poplar Road, Parkville 3052, Australia
4 Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Australia
5 Present address: DNAX Research Institute, 901 California Avenue, Palo Alto, CA 94304, USA

Correspondence to: A. M. Lew; E-mail: lew{at}wehi.edu.au

Induction of mucosal immunity, particularly to subunit vaccines, has been problematic. The primary hurdle to successful mucosal vaccination is the effective delivery of vaccine antigen to the mucosal associated lymphoid tissue. Physical and chemical barriers restrict antigen access and, moreover, immune responses induced in the mucosa can be biased towards tolerance or non-reactivity. We proposed that these difficulties could be circumvented by targeting antigen to the gastrointestinal associated lymphoid tissue via systemic (parenteral) rather than alimentary routes, using antibodies specific for the mucosal addressin cellular adhesion molecule-1 (MAdCAM). After intravenous or intramuscular injection of such rat antibodies in mice, we found a greatly enhanced (up to 3 logs) anti-rat antibody response. MAdCAM targeting induces a rapid IgA antibody response in the gut and vastly improves the systemic antibody response. Targeting also enhanced T cell proliferation and cytokine responses. Parenteral targeting of mucosal addressins may represent a generic technique for bypassing mucosal barriers and eliminating the need for adjuvants in the induction of proximal and systemic immunity.

Keywords: adhesion molecules, antigen targeting, mucosa, vaccination

Transmitting editor: A. Cooke


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
J. M. Thompson, M. G. Nicholson, A. C. Whitmore, M. Zamora, A. West, A. Iwasaki, H. F. Staats, and R. E. Johnston
Nonmucosal Alphavirus Vaccination Stimulates a Mucosal Inductive Environment in the Peripheral Draining Lymph Node
J. Immunol., July 1, 2008; 181(1): 574 - 585.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. Bourges, Y. Zhan, J. L. Brady, H. Braley, I. Caminschi, S. Prato, J. A. Villadangos, and A. M. Lew
Targeting the Gut Vascular Endothelium Induces Gut Effector CD8 T Cell Responses Via Cross-Presentation by Dendritic Cells
J. Immunol., November 1, 2007; 179(9): 5678 - 5685.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. M. Thompson, A. C. Whitmore, J. L. Konopka, M. L. Collier, E. M. B. Richmond, N. L. Davis, H. F. Staats, and R. E. Johnston
Mucosal and systemic adjuvant activity of alphavirus replicon particles
PNAS, March 7, 2006; 103(10): 3722 - 3727.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.