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International Immunology Advance Access originally published online on August 23, 2004
International Immunology 2004 16(10):1431-1437; doi:10.1093/intimm/dxh146
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© 2004 The Japanese Society for Immunology

Separation and structural analysis of lipoprotein in a lipopolysaccharide preparation from Porphyromonas gingivalis

Masahito Hashimoto, Yasuyuki Asai and Tomohiko Ogawa

Department of Oral Microbiology, Asahi University School of Dentistry, 1851-1 Hozumi, Mizuho, Gifu 501-0296, Japan

Correspondence to: T. Ogawa; E-mail: tomo527{at}dent.asahi-u.ac.jp

Lipopolysaccharide (LPS) preparations from the periodontopathic bacterium Porphyromonas gingivalis (Pg-LPS) are thought to require Toll-like receptor (TLR)2 rather than TLR4, a receptor of Escherichia coli LPS (Ec-LPS), for activation of immune cells. However, we previously reported that P. gingivalis lipid A, an immunostimulatory principal component of LPS, and its synthetic counterpart activate cells through a TLR4-dependent pathway but not via TLR2. In the present study, a lipoprotein from Pg-LPS (Pg-LP) was shown to be a principal component for TLR2-mediated cell activation. Pg-LP was separated by hydrophobic interaction chromatography followed by preparative electrophoresis and identified by internal peptide sequencing as PG1828, a putative lipoprotein encoded in the P. gingivalis genome. The N-terminal structure was characterized as a triacylated lipopeptide using mass spectrometry. Pg-LP, as well as Ec-LPS, was potent in inducing IL-8 production in human gingival fibroblasts. From our results, we propose that Pg-LP is a powerful inflammatory factor of P. gingivalis.

Keywords: Gram-negative bacteria, innate immunity, NF-{kappa}B activation, periodontal disease, Toll-like receptor

Transmitting editor: K. Sugamura


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