Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (4)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Stehling, O.
Right arrow Articles by Steiniger, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stehling, O.
Right arrow Articles by Steiniger, B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol. 16, No. 1, pp. 101-110, January 2004
© 2004 Japanese Society for Immunology

Monocyte cytotoxicity during acute kidney graft rejection in rats

Oliver Stehling1, Veronika Grau2 and Birte Steiniger1

1 Institute of Anatomy and Cell Biology, Philipps University, Robert-Koch-Strasse 6, 35033 Marburg, Germany 2 Present address: Klinik für Allgemein- und Thoraxchirurgie, Sektion Experimentelle Chirurgie, 35385 Giessen, Germany

Correspondence to: O. Stehling; E-mail: stehling{at}mailer.uni-marburg.de
Transmitting editor: T. Hünig

Surface antigens, mRNA expression patterns and intravascular accumulation suggested that monocytes might directly participate in acute renal allograft rejection in rats. A flow cytometry-based cytotoxicity assay was used in the present study to analyze the cytotoxic activity of monocytes from the renal and the whole extrapulmonary vasculature of kidney graft recipients (DA to LEW or LEW to LEW) and of untreated animals. The NK-sensitive lymphoma Yac-1 and the NO-sensitive mastocytoma P815 were labeled with FITC and used as target cells. Cellular damage was demonstrated with propidium iodide (PI) or merocyanine 540 (MC 540). On day 4 after allogeneic kidney transplantation the monocyte cytotoxicity towards Yac-1 increased 2-fold in comparison to control animals, whereas cytotoxicity towards P815 cells did not change considerably. PI and MC 540 staining indicated pro-necrotic as well as pro-apoptotic cytotoxic mechanisms. Furthermore, monocytes obviously damaged targets by cytotoxic mechanisms differing from NK cells. In conclusion, monocyte cytotoxicity might be an important effector mechanism in acute renal allograft rejection.

Keywords: apoptosis, CD161, flow cytometry, necrosis, transplantation


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
A. Hecker, Z. Mikulski, K. S. Lips, U. Pfeil, A. Zakrzewicz, S. Wilker, P. Hartmann, W. Padberg, I. Wessler, W. Kummer, et al.
Pivotal Advance: Up-regulation of acetylcholine synthesis and paracrine cholinergic signaling in intravascular transplant leukocytes during rejection of rat renal allografts
J. Leukoc. Biol., July 1, 2009; 86(1): 13 - 22.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Holler, A. Zakrzewicz, A. Kaufmann, J. Wilhelm, G. Fuchs-Moll, H. Dietrich, W. Padberg, J. Kuncova, W. Kummer, and V. Grau
Neuropeptide Y Is Expressed by Rat Mononuclear Blood Leukocytes and Strongly Down-Regulated during Inflammation
J. Immunol., November 15, 2008; 181(10): 6906 - 6912.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.