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International Immunology, Vol. 15, No. 2, pp. 159-165, February 2003
© 2003 Japanese Society for Immunology

Tracking dendritic cells: use of an in situ method to label all blood leukocytes

Bill Ristevski1, Alan J. Young2,5, Lisbet Dudler2, Ross N. P. Cahill3, Wayne Kimpton3, Elizabeth Washington3 and John B. Hay1,4

1 Department of Laboratory Medicine and Pathobiology, University of Toronto, 1 Kings’ College Circle, Toronto, Ontario M5S 1A8, Canada 2 Basel Institute for Immunology, 4005 Basel, Switzerland 3 Laboratory for Foetal and Neonatal Immunology, University of Melbourne, Parkville, Victoria 3052, Australia 4 Department of Immunology, University of Toronto, Toronto, Ontario M5S 1A8, Canada 5 Present Address: Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA

Correspondence to: J. B. Hay; E-mail: jack.hay{at}utoronto.ca
Transmitting editor: P. Ohashi

Here we describe an in situ procedure with a labeling index (percent of labeled blood leukocytes) >98%, which is high enough to permit the direct tracking of dendritic cell (DC) precursors from blood into lymphoid tissues, while circumventing the pitfalls associated with in vitro labeling. DC and lymphocytes have similar blood to afferent lymph migratory capabilities. This method has additional applications in tracking other rare cell populations in both normal and pathological states.

Keywords: carboxyfluorescein diacetate succinimidyl ester, lymph, lymph node, recirculation


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