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International Immunology, Vol. 14, No. 7, pp. 713-722, July 2002
© 2002 Japanese Society for Immunology

Exosomes bearing HLA-DR1 molecules need dendritic cells to efficiently stimulate specific T cells

Hélène Vincent-Schneider1, Pamela Stumptner-Cuvelette1, Danielle Lankar1, Sabine Pain1, Graça Raposo2, Philippe Benaroch1 and Christian Bonnerot1

1 INSERM U520, Institut Curie, 26 rue d’Ulm, 75248 Paris Cedex 05, France 2 CNRS, UMR 144, Institut Curie, 12 rue Lhomond, 75005 Paris, France

Correspondence to: C. Bonnerot; E-mail: Christian.Bonnerot{at}curie.fr
Transmitting editor: I. Pecht

Exosomes are small vesicles (60–100 nm) secreted by various cell types upon the fusion of endosomal compartments with the plasma membrane. Exosomes from antigen-presenting cells (APC), such as B lymphocytes and dendritic cells (DC), bear MHC class II molecules. In addition, the injection of DC-derived exosomes was reported to elicit potent T cell responses in vivo. Here, we analyzed the activation of specific T cells by MHC class II-bearing exosomes in vitro. The rat mast cell line, RBL-2H3, was engineered to express human class II molecules uniformly loaded with an antigenic peptide [HLA-DR1–hemagglutinin (HA)]. These cells secreted exosomes bearing DR1 class II molecules upon stimulation by a calcium ionophore or IgE receptor cross-linking. Exosomes bearing DR1–HA(306–318) complexes activated HA/DR1-specific T cells only weakly, whereas the cross-linking of such exosomes to latex beads increased stimulation of specific T cells. By contrast, the incubation of free exosomes with DC resulted in the highly efficient stimulation of specific T cells. Thus, exosomes bearing MHC class II complexes must be taken up by professional APC for efficient T cell activation.

Keywords: antigen presentation, dendritic cell, exosome, mast cell, MHC


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