Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (14)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Crepaldi, L.
Right arrow Articles by Cassatella, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Crepaldi, L.
Right arrow Articles by Cassatella, M. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol. 14, No. 10, pp. 1145-1153, October 2002
© 2002 Japanese Society for Immunology

Molecular basis of the synergistic production of IL-1 receptor antagonist by human neutrophils stimulated with IL-4 and IL-10

Luca Crepaldi1, Licia Silveri1, Federica Calzetti1, Cristina Pinardi1 and Marco A. Cassatella1

1 Department of Pathology, General Pathology Unit, University of Verona, Strada Le Grazie 4, 37134 Verona, Italy

Correspondence to: M. A. Cassatella; E-mail: marco.cassatella{at}univr.it
Transmitting editor: G. Trinchieri

In this study, we report that the release of IL-1 receptor antagonist (IL-1ra) from IL-4-stimulated neutrophils is markedly enhanced in the presence of IL-10. We also show that up-regulation of IL-1ra release by IL-10 in IL-4-stimulated neutrophils takes place through IL-1ra mRNA stabilization and enhancement of IL-1ra de novo synthesis. Furthermore, we report that the ability of IL-10 to up-regulate IL-1ra mRNA expression in IL-4-treated neutrophils requires 5–6 h and it is preceded by the acquisition of the capacity to activate Stat3 tyrosine phosphorylation. This latter response to IL-10 was strictly dependent on the levels of expression of IL-10R1, which were in fact significantly increased by IL-4 in cultured neutrophils via a signaling pathway sensitive to the serine/threonine kinase inhibitor H-7. Collectively, our data emphasize the central role of IL-10R1 expression in regulating cell responsiveness to IL-10. In addition, the fact that IL-10 strongly up-regulates IL-1ra production in IL-4-activated neutrophils uncovers a novel mechanism whereby IL-10 and IL-4 cooperate to negatively modulate the inflammatory responses.

Keywords: IL-10R1, lipopolysaccharide, STAT3


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Int ImmunolHome page
N. Tamassia, F. Calzetti, N. Menestrina, M. Rossato, F. Bazzoni, L. Gottin, and M. A. Cassatella
Circulating neutrophils of septic patients constitutively express IL-10R1 and are promptly responsive to IL-10
Int. Immunol., April 1, 2008; 20(4): 535 - 541.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
R.-O. Casasnovas, N. Mounier, P. Brice, M. Divine, F. Morschhauser, J. Gabarre, J.-Y. Blay, L. Voillat, P. Lederlin, A. Stamatoullas, et al.
Plasma Cytokine and Soluble Receptor Signature Predicts Outcome of Patients With Classical Hodgkin's Lymphoma: A Study From the Groupe d'Etude des Lymphomes de l'Adulte
J. Clin. Oncol., May 1, 2007; 25(13): 1732 - 1740.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
C. Ratthe, M. Pelletier, S. Chiasson, and D. Girard
Molecular mechanisms involved in interleukin-4-induced human neutrophils: expression and regulation of suppressor of cytokine signaling
J. Leukoc. Biol., May 1, 2007; 81(5): 1287 - 1296.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
P. M.-C. Dang, C. Elbim, J.-C. Marie, M. Chiandotto, M.-A. Gougerot-Pocidalo, and J. El-Benna
Anti-inflammatory effect of interleukin-10 on human neutrophil respiratory burst involves inhibition of GM-CSF-induced p47PHOX phosphorylation through a decrease in ERK1/2 activity
FASEB J, July 1, 2006; 20(9): 1504 - 1506.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Nolan, F. O. Maher, D. S. Martin, R. M. Clarke, M. T. Brady, A. E Bolton, K. H. G. Mills, and M. A. Lynch
Role of Interleukin-4 in Regulation of Age-related Inflammatory Changes in the Hippocampus
J. Biol. Chem., March 11, 2005; 280(10): 9354 - 9362.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
V. S. Carl, J. K. Gautam, L. D. Comeau, and M. F. Smith Jr
Role of endogenous IL-10 in LPS-induced STAT3 activation and IL-1 receptor antagonist gene expression
J. Leukoc. Biol., September 1, 2004; 76(3): 735 - 742.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
H. A. Whittington, L. Armstrong, K. M. Uppington, and A. B. Millar
Interleukin-22: A Potential Immunomodulatory Molecule in the Lung
Am. J. Respir. Cell Mol. Biol., August 1, 2004; 31(2): 220 - 226.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
A. Bouchard, C. Ratthe, and D. Girard
Interleukin-15 delays human neutrophil apoptosis by intracellular events and not via extracellular factors: role of Mcl-1 and decreased activity of caspase-3 and caspase-8
J. Leukoc. Biol., May 1, 2004; 75(5): 893 - 900.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.