International Immunology, Vol. 13, No. 5, 633-641,
May 2001
© 2001 Japanese Society for Immunology
Physical interaction of human papillomavirus virus-like particles with immune cells
1 Cancer Immunology Program, Cardinal Bernardin Cancer Center and
2 Department of Microbiology & Immunology, Loyola University Chicago, 2160 South First Avenue, Maywood, IL 60153, USA
3 Laboratory of Cellular Oncology, National Institutes of Health, 36 Convent Drive, MSC 4040, Bethesda, MD 20892-4040, USA
Correspondence to: W. M. Kast, Cardinal Bernardin Cancer Center, Loyola University Chicago, 2160 South First Avenue, Maywood, IL 60153, USA
Human papillomavirus virus-like particles (HPV VLP) and chimeric VLP are immunogens that are able to elicit potent anti-viral/tumor B and T cell responses. To investigate the immunogenicity of VLP, we determined which cells of the immune system are able to bind HPV-16 VLP. VLP were found to bind very well to human and mouse immune cells that expressed markers of antigen-presenting cells (APC) such as MHC class II, CD80 and CD86, including dendritic cells, macrophages and B cells. mAb blocking studies identified Fc
RIII (CD16) as one of the molecules to which the VLP can bind both on immune cells and foreskin epithelium. However, transfection of a CD16– cell line with CD16 did not confer binding of VLP. Splenocytes from FcRIII knockout mice showed a 33% decrease in VLP binding overall and specifically to subsets of APC. These combined data support a role for CD16 as an accessory molecule in an HPV VLP–receptor complex, possibly contributing to the immunogenicity of HPV VLP.
Keywords: antigen presentation, CD16, Fc receptor, papillomavirus, vaccine, virus-like particles
5Present address: MediGene AG, Lochhamer Strasse 11, 82152 Martinsried/Munich, Germany.
The first two authors contributed equally to this work
Transmitting editor: J. Banchereau
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