International Immunology, Vol. 13, No. 12, 1571-1581,
December 2001
© 2001 Japanese Society for Immunology
CD16+ and CD16- human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells
Department of Molecular Biomedicine, Centro de Investigación y de Estudios Avanzados, CINVESTAV-IPN, Av. IPN 2508, Mexico City 07360, Mexico.
Correspondence to: C. Sánchez-Torres
Experimental protocols for cancer immunotherapy include the utilization of autologous monocyte-derived dendritic cells (moDC) pulsed with tumor antigens. However, disease can alter the characteristics of monocyte precursors and some patients have increased numbers (up to 40%) of the minor CD16+ monocyte subpopulation, which in healthy individuals represent 10% of blood monocytes. At the present, the capacity of CD16+ monocytes to differentiate into DC has not been evaluated. Here, we investigated the ability of CD16+ monocytes cultured with granulocyte– macrophage colony-stimulating factor, IL-4 and tumor necrosis factor-
to generate DC in vitro, and we compared them to DC derived from regular CD16- monocytes. Both monocyte subsets gave rise to cells with DC characteristics. They internalized soluble and particulate antigens similarly, and both were able to stimulate T cell proliferation in autologous and allogeneic cultures. Nevertheless, CD16+ moDC expressed higher levels of CD86, CD11a and CD11c, and showed lower expression of CD1a and CD32 compared to CD16- moDC. Lipopolysaccharide-stimulated CD16- moDC expressed increased levels of IL-12 p40 mRNA and secreted greater amounts of IL-12 p70 than CD16+ moDC, whereas levels of transforming growth factor-ß1 mRNA were higher on CD16+ moDC. Moreover, CD4+ T cells stimulated with CD16+ moDC secreted increased amounts of IL-4 compared to those stimulated by CD16- moDC. These data demonstrate that both moDC are not equivalent, suggesting either that they reach different stages of maturation during the culture or that the starting monocytes belong to cell lineages with distinct differentiation capabilities.
Keywords: cellular differentiation, IL-12 secretion, monocyte subpopulations, Th1/Th2
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. P. Nishimoto, A. K. Laust, and E. L. Nelson A Human Dendritic Cell Subset Receptive to the Venezuelan Equine Encephalitis Virus-Derived Replicon Particle Constitutively Expresses IL-32 J. Immunol., September 15, 2008; 181(6): 4010 - 4018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Da Silva, S. C. Fausch, J. S. Verbeek, and W. M. Kast Uptake of Human Papillomavirus Virus-Like Particles by Dendritic Cells Is Mediated by Fc{gamma} Receptors and Contributes to Acquisition of T Cell Immunity J. Immunol., June 15, 2007; 178(12): 7587 - 7597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Ellery, E. Tippett, Y.-L. Chiu, G. Paukovics, P. U. Cameron, A. Solomon, S. R. Lewin, P. R. Gorry, A. Jaworowski, W. C. Greene, et al. The CD16+ Monocyte Subset Is More Permissive to Infection and Preferentially Harbors HIV-1 In Vivo J. Immunol., May 15, 2007; 178(10): 6581 - 6589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Ziegler-Heitbrock The CD14+ CD16+ blood monocytes: their role in infection and inflammation J. Leukoc. Biol., March 1, 2007; 81(3): 584 - 592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Ancuta, J. Wang, and D. Gabuzda CD16+ monocytes produce IL-6, CCL2, and matrix metalloproteinase-9 upon interaction with CX3CL1-expressing endothelial cells. J. Leukoc. Biol., November 1, 2006; 80(5): 1156 - 1164. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Di Pucchio, L. Pilla, I. Capone, M. Ferrantini, E. Montefiore, F. Urbani, R. Patuzzo, E. Pennacchioli, M. Santinami, A. Cova, et al. Immunization of Stage IV Melanoma Patients with Melan-A/MART-1 and gp100 Peptides plus IFN-{alpha} Results in the Activation of Specific CD8+ T Cells and Monocyte/Dendritic Cell Precursors. Cancer Res., May 1, 2006; 66(9): 4943 - 4951. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Rivas-Carvalho, M. A. Meraz-Rios, L. Santos-Argumedo, S. Bajana, G. Soldevila, M. E. Moreno-Garcia, and C. Sanchez-Torres CD16+ human monocyte-derived dendritic cells matured with different and unrelated stimuli promote similar allogeneic Th2 responses: regulation by pro- and anti-inflammatory cytokines Int. Immunol., September 1, 2004; 16(9): 1251 - 1263. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. C. Roy, G. Bandyopadhyay, S. Rakshit, M. Ray, and S. Bandyopadhyay IL-4 alone without the involvement of GM-CSF transforms human peripheral blood monocytes to a CD1adim, CD83+ myeloid dendritic cell subset J. Cell Sci., July 15, 2004; 117(16): 3435 - 3445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Turville, J. Wilkinson, P. Cameron, J. Dable, and A. L. Cunningham The role of dendritic cell C-type lectin receptors in HIV pathogenesis J. Leukoc. Biol., November 1, 2003; 74(5): 710 - 718. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Nath, F. W. Ruscetti, C. Petrow-Sadowski, and K. S. Jones Regulation of the cell-surface expression of an HTLV-I binding protein in human T cells during immune activation Blood, April 15, 2003; 101(8): 3085 - 3092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Hirst, M. S. Buzza, C. H. Bird, H. S. Warren, P. U. Cameron, M. Zhang, P. G. Ashton-Rickardt, and P. I. Bird The Intracellular Granzyme B Inhibitor, Proteinase Inhibitor 9, Is Up-Regulated During Accessory Cell Maturation and Effector Cell Degranulation, and Its Overexpression Enhances CTL Potency J. Immunol., January 15, 2003; 170(2): 805 - 815. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. Randolph, G. Sanchez-Schmitz, R. M. Liebman, and K. Schakel The CD16+ (Fc{gamma}RIII+) Subset of Human Monocytes Preferentially Becomes Migratory Dendritic Cells in a Model Tissue Setting J. Exp. Med., August 19, 2002; 196(4): 517 - 527. [Abstract] [Full Text] [PDF]
|
|