International Immunology, Vol. 12, No. 6, 807-815,
June 2000
© 2000 Japanese Society for Immunology
The adjuvant monophosphoryl lipid A increases the function of antigen-presenting cells
Département de Biologie Moléculaire, Université Libre de Bruxelles, Rue des Prof. Jeener et Brachet 12, 6041 Gosselies, Belgium
1 Smith-Kline Beecham Biologicals, Rue de l'Institut 89, 1330 Rixensart, Belgium
Correspondence to: M. Moser
The induction of immune responses in vivo is typically performed with antigens administered in external adjuvants, like alum, complete Freund's adjuvant, LPS and, more recently, monophosphoryl lipid A (MPL). However, the role of the adjuvant is still poorly defined. The aim of this study was to test whether the MPL affects the function of antigen-presenting cells (APC) in vitro and in vivo. Antigen-pulsed APC [including macrophages, B cells and dendritic cells (DC)] were incubated or not with MPL, and their ability to sensitize naive T cells was tested in vitro and in vivo. The data show that MPL enhances the ability of macrophages and B cells to sensitize naive T cells, and confers to them the capacity to induce the development of Th1 and Th2. Administration of MPL i.v. in mice results in the redistribution of fully mature DC in the T cell area of the spleen. These observations suggest that MPL may induce an antigen-specific primary immune response by provoking the migration and maturation of DC that are the physiological adjuvant of the immune system.
Keywords: adjuvant, in vivo animal models, primary response, Th1, Th2
The first two authors contributed equally to this work
Transmitting editor: J. Banchereau
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Olugbile, C. Kulangara, G. Bang, S. Bertholet, E. Suzarte, V. Villard, G. Frank, R. Audran, A. Razaname, I. Nebie, et al. Vaccine Potentials of an Intrinsically Unstructured Fragment Derived from the Blood Stage-Associated Plasmodium falciparum Protein PFF0165c Infect. Immun., December 1, 2009; 77(12): 5701 - 5709. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Didierlaurent, S. Morel, L. Lockman, S. L. Giannini, M. Bisteau, H. Carlsen, A. Kielland, O. Vosters, N. Vanderheyde, F. Schiavetti, et al. AS04, an Aluminum Salt- and TLR4 Agonist-Based Adjuvant System, Induces a Transient Localized Innate Immune Response Leading to Enhanced Adaptive Immunity J. Immunol., November 15, 2009; 183(10): 6186 - 6197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Vasilevsky, J. Colino, R. Puliaev, D. H. Canaday, and C. M. Snapper Macrophages Pulsed with Streptococcus pneumoniae Elicit a T Cell-Dependent Antibody Response upon Transfer into Naive Mice J. Immunol., August 1, 2008; 181(3): 1787 - 1797. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kool, T. Soullie, M. van Nimwegen, M. A.M. Willart, F. Muskens, S. Jung, H. C. Hoogsteden, H. Hammad, and B. N. Lambrecht Alum adjuvant boosts adaptive immunity by inducing uric acid and activating inflammatory dendritic cells J. Exp. Med., April 14, 2008; 205(4): 869 - 882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Lapteva, M. R. Seethammagari, B. A. Hanks, J. Jiang, J. M. Levitt, K. M. Slawin, and D. M. Spencer Enhanced Activation of Human Dendritic Cells by Inducible CD40 and Toll-like Receptor-4 Ligation Cancer Res., November 1, 2007; 67(21): 10528 - 10537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Bevaart, M. J.H. Jansen, M. J. van Vugt, J. S. Verbeek, J. G.J. van de Winkel, and J. H.W. Leusen The High-Affinity IgG Receptor, Fc{gamma}RI, Plays a Central Role in Antibody Therapy of Experimental Melanoma Cancer Res., February 1, 2006; 66(3): 1261 - 1264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. S. Thompson, P. M. Chilton, J. R. Ward, J. T. Evans, and T. C. Mitchell The low-toxicity versions of LPS, MPL(R) adjuvant and RC529, are efficient adjuvants for CD4+ T cells J. Leukoc. Biol., December 1, 2005; 78(6): 1273 - 1280. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Luxameechanporn, V. Kirtsreesakul, J. Klemens, P. Khoury, K. Thompson, and R. M. Naclerio Evaluation of Importance of Toll-like Receptor 4 in Acute Streptococcus pneumoniae Sinusitis in Mice Arch Otolaryngol Head Neck Surg, November 1, 2005; 131(11): 1001 - 1006. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Liu, X. Gu, J. Scafidi, and A. E. Davis III N-Linked Glycosylation Is Required for C1 Inhibitor-Mediated Protection from Endotoxin Shock in Mice Infect. Immun., April 1, 2004; 72(4): 1946 - 1955. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Martin, S. M. Michalek, and J. Katz Role of Innate Immune Factors in the Adjuvant Activity of Monophosphoryl Lipid A Infect. Immun., May 1, 2003; 71(5): 2498 - 2507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q.-B. Yang, M. Martin, S. M. Michalek, and J. Katz Mechanisms of Monophosphoryl Lipid A Augmentation of Host Responses to Recombinant HagB from Porphyromonas gingivalis Infect. Immun., July 1, 2002; 70(7): 3557 - 3565. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. D. Hawkins, S. T. Ishizaka, P. McGuinness, H. Zhang, W. Gavin, B. DeCosta, Z. Meng, H. Yang, M. Mullarkey, D. W. Young, et al. A Novel Class of Endotoxin Receptor Agonists with Simplified Structure, Toll-Like Receptor 4-Dependent Immunostimulatory Action, and Adjuvant Activity J. Pharmacol. Exp. Ther., February 1, 2002; 300(2): 655 - 661. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ismaili, J. Rennesson, E. Aksoy, J. Vekemans, B. Vincart, Z. Amraoui, F. Van Laethem, M. Goldman, and P. M. Dubois Monophosphoryl Lipid A Activates Both Human Dendritic Cells and T Cells J. Immunol., January 15, 2002; 168(2): 926 - 932. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Alexander and E. Th. Rietschel Invited review: Bacterial lipopolysaccharides and innate immunity Innate Immunity, June 1, 2001; 7(3): 167 - 202. [Abstract] [PDF]
|
|