International Immunology, Vol. 12, No. 4, 547-554,
April 2000
© 2000 Japanese Society for Immunology
Stimulation of Fc
R receptors induces monocyte chemoattractant protein-1 in the human monocytic cell line THP-1 by a mechanism involving I
B-
degradation and formation of p50/p65 NF-B/Rel complexes
Instituto de Biología y Genética Molecular, Consejo Superior de Investigaciones Científicas, Facultad de Medicina, 47005 Valladolid, Spain
Correspondence to: M. Sánchez Crespo
THP-1 monocytic/macrophage cells were stimulated via their Fc
R receptors with insoluble aggregates of human IgG and the production of the C–C chemokine monocyte chemoattractant protein (MCP)-1 assayed. A dose- and time-dependent production of MCP-1 comparable to that produced by the most potent agonists could be detected in the culture medium by a sensitive ELISA assay. This was accompanied by a parallel activation of the transcription factor NF-
B as judged from both the appearance of B-binding activity containing p50/p65 NF-B/Rel complexes in the nuclear extract and the disappearance of the NF-B inhibitor IB-
in the cell lysate. In contrast, IB-ß and IB-
expression was not modified, thus pointing to the occurrence of a selective degradation of IB- under those conditions. Attempts to modulate MCP-1 production with compounds that display inhibitory effects on the activation of NF-B such as the proteasome inhibitor N-acetyl-leucinyl-leucinyl-norleucinal, the antioxidant pyrrolidine dithiocarbamate and the salicylate derivative 2-hydroxy-4-trifluoromethylbenzoic acid showed a parallel effect on both MCP-1 production and NF-B activation, thus pointing to the involvement of B-binding sites on the transcriptional regulation of MCP-1 production. Our findings suggest the existence in monocytic cells of a signaling mechanism initiated by cross-linking of low-affinity FcR, most likely of the FcRII family since THP-1 cells do not express FcRIII receptors, that involves activation of NF-B associated to the proteolytic degradation of IB- and leads to the transcriptional up-regulation of MCP-1.
Keywords: chemokines, Fc receptors, inflammation, monocytes/macrophages, transcription factors
Transmitting editor: E. R. Unanue
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. G. Trinidad, M. L. de la Puerta, N. Fernandez, Y. Bayon, M. S. Crespo, and A. Alonso Coupling of C3bi to IgG inhibits the tyrosine phosphorylation signaling cascade downstream Syk and reduces cytokine induction in monocytes J. Leukoc. Biol., May 1, 2006; 79(5): 1073 - 1082. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Guizani-Tabbane, K. Ben-Aissa, M. Belghith, A. Sassi, and K. Dellagi Leishmania major Amastigotes Induce p50/c-Rel NF-{kappa}B Transcription Factor in Human Macrophages: Involvement in Cytokine Synthesis Infect. Immun., May 1, 2004; 72(5): 2582 - 2589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Eglite, J. M. Morin, and H. Metzger Synthesis and Secretion of Monocyte Chemotactic Protein-1 Stimulated by the High Affinity Receptor for IgE J. Immunol., March 1, 2003; 170(5): 2680 - 2687. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Colmenares, S. L. Constant, P. E. Kima, and D. McMahon-Pratt Leishmania pifanoi Pathogenesis: Selective Lack of a Local Cutaneous Response in the Absence of Circulating Antibody Infect. Immun., December 1, 2002; 70(12): 6597 - 6605. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Fuentes, M. Hernandez, F. J. Fernandez-Aviles, M. S. Crespo, and M. L. Nieto Cooperation Between Secretory Phospholipase A2 and TNF-Receptor Superfamily Signaling: Implications for the Inflammatory Response in Atherogenesis Circ. Res., October 18, 2002; 91(8): 681 - 688. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Drechsler, S. Chavan, D. Catalano, P. Mandrekar, and G. Szabo Fc{gamma}R cross-linking mediates NF-{kappa}B activation, reduced antigen presentation capacity, and decreased IL-12 production in monocytes without modulation of myeloid dendritic cell development J. Leukoc. Biol., October 1, 2002; 72(4): 657 - 667. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Fernandez, M. Renedo, C. Garcia-Rodriguez, and M. Sanchez Crespo Activation of Monocytic Cells Through Fc{gamma} Receptors Induces the Expression of Macrophage-Inflammatory Protein (MIP)-1{alpha}, MIP-1{beta}, and RANTES J. Immunol., September 15, 2002; 169(6): 3321 - 3328. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. TEGEDER, J. PFEILSCHIFTER, and G. GEISSLINGER Cyclooxygenase-independent actions of cyclooxygenase inhibitors FASEB J, October 1, 2001; 15(12): 2057 - 2072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hernandez, L. Fuentes, F. J. Fernandez Aviles, M. S. Crespo, and M. L. Nieto Secretory Phospholipase A2 Elicits Proinflammatory Changes and Upregulates the Surface Expression of Fas Ligand in Monocytic Cells: Potential Relevance for Atherogenesis Circ. Res., January 11, 2002; 90(1): 38 - 45. [Abstract] [Full Text] [PDF]
|
|