International Immunology, Vol. 12, No. 11, 1511-1519,
November 2000
© 2000 Japanese Society for Immunology
Expression of multilectin receptors and comparative FITC–dextran uptake by human dendritic cells
Haematology/Immunology Research Group, Christchurch School of Medicine, Christchurch, New Zealand
1 Mater Medical Research Institute, Level 3, Aubigny Place, Raymond Terrace, South Brisbane, Queensland 4101, Australia
Correspondence to: M. Kato
Dendritic cells (DC) are potent antigen-presenting cells and understanding their mechanisms of antigen uptake is important for loading DC with antigen for immunotherapy. The multilectin receptors, DEC-205 and macrophage mannose receptor (MMR), are potential antigen-uptake receptors; therefore, we examined their expression and FITC–dextran uptake by various human DC preparations. The RT-PCR analysis detected low levels of DEC-205 mRNA in immature blood DC, Langerhans cells (LC) and immature monocyte-derived DC (Mo-DC). Its mRNA expression increased markedly upon activation, indicating that DEC-205 is an activation-associated molecule. In Mo-DC, the expression of cell-surface DEC-205 increased markedly during maturation. In blood DC, however, the cell-surface expression of DEC-205 did not change during activation, suggesting the presence of a large intracellular pool of DEC-205 or post-transcriptional regulation. Immature Mo-DC expressed abundant MMR, but its expression diminished upon maturation. Blood DC and LC did not express detectable levels of the MMR. FITC–dextran uptake by both immature and activated blood DC was 30- to 70-fold less than that of LC, immature Mo-DC and macrophages. In contrast to immature Mo-DC, the FITC–dextran uptake by LC was not inhibited effectively by mannose, an inhibitor for MMR-mediated FITC–dextran uptake. Thus, unlike Mo-DC, blood DC and LC do not use the MMR for carbohydrate-conjugated antigen uptake and alternative receptors may yet be defined on these DC. Therefore, DEC-205 may have a different specificity as an antigen uptake receptor or contribute to an alternative DC function.
Keywords: DEC-205, Langerhans cells, macrophage mannose receptor, monocyte-derived dendritic cells
2 Current address: Department of Dermatology, University of Würzburg, 97080 Würzburg, Germany
Transmission editor: A. McMichael
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