International Immunology, Vol. 11, No. 8, 1195-1202,
August 1999
© 1999 Japanese Society for Immunology
5 is required for rearrangement of the Ig 
light chain gene in pro-B cell lines
1 Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama226-8501, Japan
2 Department of Immunology, The Tokyo Metropolitan Institute of Medical Science, Tokyo, 113–8613 Japan
Correspondence to: A. Kudo
5 associates with Vpre-B to form the surrogate light (L) chain. The phenotype of 5 knockout mice showed severe impairment of B cell development from pro-B to immature B cell stages. To investigate the function of the surrogate L chain at this stage, we restored expression of 5 to 5-deficient pro-B cell lines which were established from bone marrow cells of 5 knockout mice in the presence of IL-7 and a stromal cell line. Some of these lines are severely impaired in B cell development from pro-B to immature B cell stages as is seen in vivo in 5 knockout mice. Restoration of 5 protein by retroviral-mediated gene transfer into established 5-deficient pro-B cell lines induced rearrangement of the Ig 
L chain genes after removal of IL-7 from the culture. Immunoprecipitation revealed that the restored 5 in the cell line is coupled with Vpre-B to form the surrogate L chain. The results demonstrate that formation of a complete surrogate L chain, consisting of both 5 and Vpre-B, stimulates efficient rearrangement of the L chain genes.
Keywords: light chain, 5, rearrangement, surrogate light chain, Vpre-B
Transmitting editor: D. Kitamura
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