International Immunology, Vol. 11, No. 7, 1139-1147,
July 1999
© 1999 Japanese Society for Immunology
CD40 signaling induces B cell responsiveness to multiple members of the 
chain-common cytokine family
Veterinary Infectious Disease Organization, 120 Veterinary Road, Saskatoon, Saskatchewan S7N 5E3, Canada
1 Institut Bordet, University of Brussels, 121 Boulevard de Waterloo, 1000 Brussels, Belgium
2 Basel Institute for Immunology, 487 Grenzacherstrasse, Basel 4005, Switzerland
Correspondence to: P. J. Griebel
CD40 signaling induces B cell proliferative and differentiation responses that can be modulated by many different cytokines. Cytokines in the IL-2 receptor 
chain (c)-common family are known to play an integral role in B cell development. Therefore, we investigated the possibility that CD40 signaling induced B cell responsiveness to multiple c-common cytokines and that individual c-common cytokines induced distinct B cell responses. B cells were isolated from lymphoid follicles of sheep Peyer's patches (PP) and co-cultured with murine CD40 ligand (mCD40L). CD40 signaling induced PP B cell responsiveness to recombinant human IL-2, IL-4, IL-7 and IL-15. mCD40L-induced B cell growth was enhanced by combining IL-4 with a second c-common cytokine and sustained B cell growth required co-stimulation with IL-4 plus IL-2, IL-7 and IL-15. c-common cytokine responsiveness remained dependent upon CD40 signaling, and removal of mCD40L resulted in B cell differentiation and cell death. Similar proliferative responses to mCD40L and c-common cytokines were observed for both immature (ileal) and mature (jejunal) PP B cells. Finally, the capacity of CD40-activated B cells to respond to multiple c-common cytokines was analyzed with individual PP B cell clones. All B cell clones displayed similar proliferative responses to IL-2 but quantitatively different responses to IL-4, IL-7 and IL-15. The biological significance of B cell responsiveness to multiple c-common cytokines is discussed.
Keywords: B lymphocytes, CD40, CD40 ligand, chain-common cytokines, IL-2, IL-4, IL-7, IL-15, Peyer's patch, sheep
Transmitting editor: C. J. Paige
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Klener, M. Szynal, Y. Cleuter, M. Merimi, H. Duvillier, F. Lallemand, C. Bagnis, P. Griebel, C. Sotiriou, A. Burny, et al. Insights into Gene Expression Changes Impacting B-Cell Transformation: Cross-Species Microarray Analysis of Bovine Leukemia Virus Tax-Responsive Genes in Ovine B Cells J. Virol., February 15, 2006; 80(4): 1922 - 1938. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Van den Broeke, Y. Cleuter, T. Beskorwayne, P. Kerkhofs, M. Szynal, C. Bagnis, A. Burny, and P. Griebel CD154 Costimulated Ovine Primary B Cells, a Cell Culture System That Supports Productive Infection by Bovine Leukemia Virus J. Virol., February 1, 2001; 75(3): 1095 - 1103. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. Flaishon, R. Hershkoviz, F. Lantner, O. Lider, R. Alon, Y. Levo, R. A. Flavell, and I. Shachar Autocrine Secretion of Interferon {gamma} Negatively Regulates Homing of Immature B Cells J. Exp. Med., November 6, 2000; 192(9): 1381 - 1388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hiroi, M. Yanagita, N. Ohta, G. Sakaue, and H. Kiyono IL-15 and IL-15 Receptor Selectively Regulate Differentiation of Common Mucosal Immune System-Independent B-1 Cells for IgA Responses J. Immunol., October 15, 2000; 165(8): 4329 - 4337. [Abstract] [Full Text] [PDF]
|
|