International Immunology, Vol. 11, No. 5, 731-738,
May 1999
© 1999 Japanese Society for Immunology
Identification of an 
2,6-sialyltransferase induced early after lymphocyte activation
Institute for Medical Microbiology and Hygiene, Department of Immunology, University of Freiburg, Hermann-Herder-Strasse 11, 79104 Freiburg, Germany
1 Molecular Glycobiology, Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN), Wako, Saitama 351-0198, Japan
2 Tumor Immunology program, German Cancer Research Center, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
Correspondence to: H. Pircher
We have used mRNA differential display PCR to search for genes induced in activated T cells and we identified a gene encoding an 
2,6-sialyltransferase (ST6GalNAc IV) that is rapidly induced in lymphocytes after antigen or mitogen stimulation. The 3.6 kb full-length cDNA clone (MK45) obtained contained a single open reading frame encoding a 302 amino acid protein and a 2.5 kb 3' untranslated region. MK45 expression in in vivo-activated CD8 T cells reached the highest level 4 h after antigen triggering and then declined rapidly to nearly base levels within 45 h. Northern blot analysis further revealed that MK45 expression was also induced in LPS-activated B cells and antigen-triggered CD4 T cells in vitro. MK45 expression was low or undetectable in most other mouse tissues examined, when compared to activated lymphocytes. Importantly, the mRNA expression level of other sialyltransferases remained largely unchanged during the early stage of lymphocyte activation. Finally, increased ecto-sialyltransferase activity and an altered sialylation pattern were demonstrated on the cell surface of early activated CD8 T cells. Our report identifies a candidate sialyltransferase gene that is involved in the early alteration of the sialylation pattern of cell surface molecules in activated lymphocytes.
Keywords: differential display, glycosyltransferases, lymphocyte stimulation, murine, sialylation
Transmitting editor: K. Eichmann
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Rifat, T. J. Kang, D. Mann, L. Zhang, A. C. Puche, N. M. Stamatos, S. E. Goldblum, R. Brossmer, and A. S. Cross Expression of sialyltransferase activity on intact human neutrophils J. Leukoc. Biol., October 1, 2008; 84(4): 1075 - 1081. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Hernandez, J. Klein, S. J. Van Dyken, J. D. Marth, and L. G. Baum T-cell activation results in microheterogeneous changes in glycosylation of CD45 Int. Immunol., July 2, 2007; (2007) dxm053v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Comelli, M. Sutton-Smith, Q. Yan, M. Amado, M. Panico, T. Gilmartin, T. Whisenant, C. M. Lanigan, S. R. Head, D. Goldberg, et al. Activation of Murine CD4+ and CD8+ T Lymphocytes Leads to Dramatic Remodeling of N-Linked Glycans J. Immunol., August 15, 2006; 177(4): 2431 - 2440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Stocker and E. S. Bennett Differential Sialylation Modulates Voltage-gated Na+ Channel Gating throughout the Developing Myocardium J. Gen. Physiol., February 27, 2006; 127(3): 253 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kao, M. A. Daniels, and S. C. Jameson Loss of CD8 and TCR binding to Class I MHC ligands following T cell activation Int. Immunol., December 1, 2005; 17(12): 1607 - 1617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Morgan, G. Gao, J. Pawling, J. W. Dennis, M. Demetriou, and B. Li N-Acetylglucosaminyltransferase V (Mgat5)-Mediated N-Glycosylation Negatively Regulates Th1 Cytokine Production by T Cells J. Immunol., December 15, 2004; 173(12): 7200 - 7208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lanteri, V. Giordanengo, N. Hiraoka, J.-G. Fuzibet, P. Auberger, M. Fukuda, L. G. Baum, and J.-C. Lefebvre Altered T cell surface glycosylation in HIV-1 infection results in increased susceptibility to galectin-1-induced cell death Glycobiology, December 1, 2003; 13(12): 909 - 918. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Amano, M. Galvan, J. He, and L. G. Baum The ST6Gal I Sialyltransferase Selectively Modifies N-Glycans on CD45 to Negatively Regulate Galectin-1-induced CD45 Clustering, Phosphatase Modulation, and T Cell Death J. Biol. Chem., February 21, 2003; 278(9): 7469 - 7475. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Hernandez and L. G. Baum Ah, sweet mystery of death! Galectins and control of cell fate Glycobiology, October 1, 2002; 12(10): 127R - 136R. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Onami, L. E. Harrington, M. A. Williams, M. Galvan, C. P. Larsen, T. C. Pearson, N. Manjunath, L. G. Baum, B. D. Pearce, and R. Ahmed Dynamic Regulation of T Cell Immunity by CD43 J. Immunol., June 15, 2002; 168(12): 6022 - 6031. [Abstract] [Full Text] [PDF]
|
|