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International Immunology, Vol. 11, No. 2, 243-247, February 1999
© 1999 Japanese Society for Immunology

Marking IL-4-producing cells by knock-in of the IL-4 gene

I-Cheng Ho1,2, Mark H. Kaplan1, Laurie Jackson-Grusby3, Laurie H. Glimcher1,2 and Michael J. Grusby1,2

1 Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA
2 Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
3 The Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA

Correspondence to: M. J. Grusby, Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA

IL-4 is a cytokine which can be expressed by a number of cell types including Th2 cells, mast cells and a population of CD4+ NK1.1+ NK T cells. Although phenotypic markers exist for identifying each of these cell types, there is at present no known cell surface marker common to all IL-4-producing cells. Using gene targeting in embryonic stem cells, we have modified the IL-4 locus by knock-in of a transmembrane domain to generate mice that express a membrane-bound form of IL-4 (mIL-4). Flow cytometry using an IL-4-specific mAb allowed the detection of IL-4-secreting Th2 cells, mast cells and NK T cells from mIL-4 mice. Furthermore, the analysis of immune responses in mIL-4 mice following immunization with anti-CD3 and anti-IgD has allowed us to identify distinct subpopulations of IL-4-producing NK T cells. Thus, the expression of IL-4 in a membrane-bound form provides a novel method for the identification and characterization of IL-4-producing cells.

Keywords: cytokines, Th1/Th2, transgenic/knockout

Transmitting editor: A. Singer


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