International Immunology, Vol 10, 991-998, Copyright © 1998 by Oxford University Press
YC Zang, M Kozovska, I Aebischer, S Li, S Boehme, P Crowe, VM Rivera and JZ Zhang
T cell responses to myelin basic protein (MBP) are thought to play an
important role in the pathogenesis of multiple sclerosis (MS). The response
to the 83-99 region of MBP represents a dominant response to MBP in
patients with MS and is associated with HLA-DR2 that is linked with
susceptibility to MS. Although T cell clones reactive to various regions of
MBP have been found to exhibit heterogeneous TCR Vbeta gene usage in
patients with MS, it is unclear whether T cell clones uniformly recognizing
the 83-99 peptide of MBP in the context of the same DR molecule would have
restricted TCR V gene rearrangements and recognition motifs. In this study,
a panel of DR2- or DR4-restricted T cell clones specific for the MBP83-99
peptide were derived from 11 patients with MS and examined for TCR V gene
usage by PCR and the recognition motifs using analog peptides. Our study
revealed that despite a few T cell clone pairs having similar recognition
motifs and shared sequence homology in the CDR3, the overall recognition
motifs of MBP83-99-specific T cells were considerably diverse.
Interestingly, the DR2-restricted T cell clones displayed a biased V gene
usage for Valpha3 and Valpha8, while Vbeta gene rearrangements were highly
heterogeneous. This study provided experimental evidence suggesting a
limited heterogeneity in TCR Valpha gene rearrangements of MBP-reactive T
cells in DR2 patients with MS.
ARTICLES
Restricted TCR Valpha gene rearrangements in T cells recognizing an immunodominant peptide of myelin basic protein in DR2 patients with multiple sclerosis
Department of Neurology and Baylor-Methodist International Multiple Sclerosis Center, Baylor College of Medicine, Veterans Affairs Medical Center, Houston, TX 77030, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. P. Mycko, H. Waldner, D. E. Anderson, K. D. Bourcier, K. W. Wucherpfennig, V. K. Kuchroo, and D. A. Hafler Cross-Reactive TCR Responses to Self Antigens Presented by Different MHC Class II Molecules J. Immunol., August 1, 2004; 173(3): 1689 - 1698. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hong, Y. C. Q. Zang, M. V. Tejada-Simon, S. Li, V. M. Rivera, J. Killian, and J. Z. Zhang Reactivity and Regulatory Properties of Human Anti-Idiotypic Antibodies Induced by T Cell Vaccination J. Immunol., December 15, 2000; 165(12): 6858 - 6864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. C. Q. Zang, J. Hong, V. M. Rivera, J. Killian, and J. Z. Zhang Preferential Recognition of TCR Hypervariable Regions by Human Anti-Idiotypic T Cells Induced by T Cell Vaccination J. Immunol., April 15, 2000; 164(8): 4011 - 4017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hong, Y. C. Q. Zang, M. V. Tejada-Simon, M. Kozovska, S. Li, R. A. K. Singh, D. Yang, V. M. Rivera, J. K. Killian, and J. Z. Zhang A Common TCR V-D-J Sequence in V{beta}13.1 T Cells Recognizing an Immunodominant Peptide of Myelin Basic Protein in Multiple Sclerosis J. Immunol., September 15, 1999; 163(6): 3530 - 3538. [Abstract] [Full Text] [PDF]
|
|